dc.contributor.author | Ferlez, BH | |
dc.contributor.author | Kirst, H | |
dc.contributor.author | Greber, BJ | |
dc.contributor.author | Nogales, E | |
dc.contributor.author | Sutter, M | |
dc.contributor.author | Kerfeld, CA | |
dc.coverage.spatial | Germany | |
dc.date.accessioned | 2023-06-07T09:49:28Z | |
dc.date.available | 2023-06-07T09:49:28Z | |
dc.date.issued | 2023-03-18 | |
dc.identifier.citation | Advanced Materials, 2023, pp. e2212065 - | en_US |
dc.identifier.issn | 0935-9648 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5831 | |
dc.identifier.eissn | 1521-4095 | |
dc.identifier.eissn | 1521-4095 | |
dc.identifier.doi | 10.1002/adma.202212065 | |
dc.description.abstract | Many bacteria use protein-based organelles known as bacterial microcompartments (BMCs) to organize and sequester sequential enzymatic reactions. Regardless of their specialized metabolic function, all BMCs are delimited by a shell made of multiple structurally redundant, yet functionally diverse, hexameric (BMC-H), pseudohexameric/trimeric (BMC-T), or pentameric (BMC-P) shell protein paralogs. When expressed without their native cargo, shell proteins have been shown to self-assemble into 2D sheets, open-ended nanotubes, and closed shells of ≈40 nm diameter that are being developed as scaffolds and nanocontainers for applications in biotechnology. Here, by leveraging a strategy for affinity-based purification, it is demonstrated that a wide range of empty synthetic shells, many differing in end-cap structures, can be derived from a glycyl radical enzyme-associated microcompartment. The range of pleomorphic shells observed, which span ≈2 orders of magnitude in size from ≈25 nm to ≈1.8 µm, reveal the remarkable plasticity of BMC-based biomaterials. In addition, new capped nanotube and nanocone morphologies are observed that are consistent with a multicomponent geometric model in which architectural principles are shared among asymmetric carbon, viral protein, and BMC-based structures. | |
dc.format | Print-Electronic | |
dc.format.extent | e2212065 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
dc.relation.ispartof | Advanced Materials | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.subject | bacterial microcompartments | |
dc.subject | fullerenes | |
dc.subject | nanocones | |
dc.subject | nanotubes | |
dc.subject | self-assembly | |
dc.subject | synthetic biology | |
dc.title | Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano- to Microscale. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2023-03-18 | |
dc.date.updated | 2023-06-07T09:47:54Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1002/adma.202212065 | en_US |
rioxxterms.licenseref.startdate | 2023-03-18 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/36932732 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Structural biology of DNA repair complexes | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1002/adma.202212065 | |
icr.researchteam | Struct Biol DNA repair | en_US |
dc.contributor.icrauthor | Greber, Basil | |
icr.provenance | Deposited by Mr Arek Surman on 2023-06-07. Deposit type is initial. No. of files: 1. Files: Advanced Materials - 2023 - Ferlez - Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures.pdf | |