dc.contributor.author | Pollock, K | |
dc.contributor.author | Ranes, M | |
dc.contributor.author | Collins, I | |
dc.contributor.author | Guettler, S | |
dc.date.accessioned | 2017-08-23T09:07:29Z | |
dc.date.issued | 2017-01-01 | |
dc.identifier.citation | Methods in molecular biology (Clifton, N.J.), 2017, 1608 pp. 445 - 473 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/790 | |
dc.identifier.eissn | 1940-6029 | |
dc.identifier.doi | 10.1007/978-1-4939-6993-7_28 | |
dc.description.abstract | The poly(ADP-ribose)polymerase (PARP) enzyme tankyrase (TNKS/ARTD5, TNKS2/ARTD6) uses its ankyrin repeat clusters (ARCs) to recognize degenerate peptide motifs in a wide range of proteins, thereby recruiting such proteins and their complexes for scaffolding and/or poly(ADP-ribosyl)ation. Here, we provide guidance for predicting putative tankyrase-binding motifs, based on the previously delineated peptide sequence rules and existing structural information. We present a general method for the expression and purification of tankyrase ARCs from Escherichia coli and outline a fluorescence polarization assay to quantitatively assess direct ARC-TBM peptide interactions. We provide a basic protocol for evaluating binding and poly(ADP-ribosyl)ation of full-length candidate interacting proteins by full-length tankyrase in mammalian cells. | |
dc.format | Print | |
dc.format.extent | 445 - 473 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | Springer New York | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Telomere | |
dc.subject | Animals | |
dc.subject | Humans | |
dc.subject | Tankyrases | |
dc.subject | Binding Sites | |
dc.subject | Protein Binding | |
dc.subject | Poly ADP Ribosylation | |
dc.title | Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-01-01 | |
rioxxterms.versionofrecord | 10.1007/978-1-4939-6993-7_28 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2017-01 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Methods in molecular biology (Clifton, N.J.) | |
pubs.notes | No embargo | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Medicinal Chemistry 2 | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Structural Biology of Cell Signalling | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Medicinal Chemistry 2 | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Structural Biology of Cell Signalling | |
pubs.publication-status | Published | |
pubs.volume | 1608 | |
pubs.embargo.terms | No embargo | |
icr.researchteam | Medicinal Chemistry 2 | |
icr.researchteam | Structural Biology of Cell Signalling | |
dc.contributor.icrauthor | Pollock, Katie | |
dc.contributor.icrauthor | Collins, Ian | |
dc.contributor.icrauthor | Guettler, Sebastian | |