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dc.contributor.authorAbascal-Palacios, Gen_US
dc.contributor.authorRamsay, EPen_US
dc.contributor.authorBeuron, Fen_US
dc.contributor.authorMorris, Een_US
dc.contributor.authorVannini, Aen_US
dc.coverage.spatialEnglanden_US
dc.date.accessioned2018-01-15T14:24:58Z
dc.date.issued2018-01-17en_US
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/29345637en_US
dc.identifiernature25441en_US
dc.identifier.citationNature, 2018, 553 (7688), pp. 301 - 306en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/998
dc.identifier.eissn1476-4687en_US
dc.identifier.doi10.1038/nature25441en_US
dc.description.abstractRNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene transcription by Pol III requires the activity of the transcription factor TFIIIB to form a transcriptionally active Pol III preinitiation complex (PIC). Here we present electron microscopy reconstructions of Pol III PICs at 3.4-4.0 Å and a reconstruction of unbound apo-Pol III at 3.1 Å. TFIIIB fully encircles the DNA and restructures Pol III. In particular, binding of the TFIIIB subunit Bdp1 rearranges the Pol III-specific subunits C37 and C34, thereby promoting DNA opening. The unwound DNA directly contacts both sides of the Pol III cleft. Topologically, the Pol III PIC resembles the Pol II PIC, whereas the Pol I PIC is more divergent. The structures presented unravel the molecular mechanisms underlying the first steps of Pol III transcription and also the general conserved mechanisms of gene transcription initiation.en_US
dc.format.extent301 - 306en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_US
dc.subjectCryoelectron Microscopyen_US
dc.subjectDNAen_US
dc.subjectModels, Molecularen_US
dc.subjectNucleic Acid Conformationen_US
dc.subjectPromoter Regions, Geneticen_US
dc.subjectProtein Subunitsen_US
dc.subjectRNA Polymerase Ien_US
dc.subjectRNA Polymerase IIen_US
dc.subjectRNA Polymerase IIIen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectSaccharomyces cerevisiae Proteinsen_US
dc.subjectTemplates, Geneticen_US
dc.subjectTranscription Factor TFIIIBen_US
dc.subjectTranscription Factors, TFIIen_US
dc.subjectTranscription Initiation, Geneticen_US
dc.titleStructural basis of RNA polymerase III transcription initiation.en_US
dc.typeJournal Article
dcterms.dateAccepted2017-12-08en_US
rioxxterms.versionofrecord10.1038/nature25441en_US
rioxxterms.licenseref.startdate2018-01-17en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfNatureen_US
pubs.issue7688en_US
pubs.notes6 monthsen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Structural Electron Microscopy
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Vannini Group
pubs.publication-statusPublisheden_US
pubs.volume553en_US
pubs.embargo.terms6 monthsen_US
icr.researchteamStructural Electron Microscopyen_US
icr.researchteamVannini Groupen_US
dc.contributor.icrauthorVannini, Alessandroen_US
dc.contributor.icrauthorBeuron, Fabienneen_US
dc.contributor.icrauthorMorris, Edwarden_US
dc.contributor.icrauthorAbascal Palacios, Guillermoen_US


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