Browsing ICR Divisions by author "Choudhary, Jyoti"
Now showing items 1-20 of 87
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3D Functional Genomics Screens Identify CREBBP as a Targetable Driver in Aggressive Triple-Negative Breast Cancer.
Peck, B; Bland, P; Mavrommati, I; Muirhead, G; Cottom, H; et al. (AMER ASSOC CANCER RESEARCH, 2021-02-15)Triple-negative breast cancers (TNBC) are resistant to standard-of-care chemotherapy and lack known targetable driver gene alterations. Identification of novel drivers could aid the discovery of new treatment strategies ... -
A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.
Cutts, EE; Taylor, GC; Pardo, M; Yu, L; Wills, JC; et al. (F1000 Research Ltd, 2021-01-13)Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. ... -
A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes.
Schlaffner, CN; Pirklbauer, GJ; Bender, A; Steen, JAJ; Choudhary, JS (2018-05-22)Cross-talk between genes, transcripts, and proteins is the key to cellular responses; hence, analysis of molecular levels as distinct entities is slowly being extended to integrative studies to enhance the understanding ... -
A Knockout Screen of ApiAP2 Genes Reveals Networks of Interacting Transcriptional Regulators Controlling the Plasmodium Life Cycle.
Modrzynska, K; Pfander, C; Chappell, L; Yu, L; Suarez, C; et al. (CELL PRESS, 2017-01-11)A family of apicomplexa-specific proteins containing AP2 DNA-binding domains (ApiAP2s) was identified in malaria parasites. This family includes sequence-specific transcription factors that are key regulators of development. ... -
A molecular quantitative trait locus map for osteoarthritis.
Steinberg, J; Southam, L; Roumeliotis, TI; Clark, MJ; Jayasuriya, RL; et al. (NATURE PORTFOLIO, 2021-02-26)Osteoarthritis causes pain and functional disability for over 500 million people worldwide. To develop disease-stratifying tools and modifying therapies, we need a better understanding of the molecular basis of the disease ... -
A quantitative proteomic screen of the Campylobacter jejuni flagellar-dependent secretome.
Scanlan, E; Yu, L; Maskell, D; Choudhary, J; Grant, A (ELSEVIER SCIENCE BV, 2017-01-30)UNLABELLED: Campylobacter jejuni is the leading cause of bacterial gastroenteritis in the world. A number of factors are believed to contribute to the ability of C. jejuni to cause disease within the human host including ... -
Activation of the Aryl Hydrocarbon Receptor Interferes with Early Embryonic Development.
Gialitakis, M; Tolaini, M; Li, Y; Pardo, M; Yu, L; et al. (CELL PRESS, 2017-10-01)The transcriptional program of early embryonic development is tightly regulated by a set of well-defined transcription factors that suppress premature expression of differentiation genes and sustain the pluripotent identity. ... -
An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules.
Bakos, G; Yu, L; Gak, IA; Roumeliotis, TI; Liakopoulos, D; et al. (NATURE PUBLISHING GROUP, 2018-11-14)Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck ... -
Aneuploidy tolerance caused by BRG1 loss allows chromosome gains and recovery of fitness.
Schiavoni, F; Zuazua-Villar, P; Roumeliotis, TI; Benstead-Hume, G; Pardo, M; et al. (NATURE PORTFOLIO, 2022-04-01)Aneuploidy results in decreased cellular fitness in many species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth, suggesting that the impact of aneuploidy ... -
Arc Requires PSD95 for Assembly into Postsynaptic Complexes Involved with Neural Dysfunction and Intelligence.
Fernández, E; Collins, MO; Frank, RAW; Zhu, F; Kopanitsa, MV; et al. (CELL PRESS, 2017-10-17)Arc is an activity-regulated neuronal protein, but little is known about its interactions, assembly into multiprotein complexes, and role in human disease and cognition. We applied an integrated proteomic and genetic ... -
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Liess, AKL; Kucerova, A; Schweimer, K; Yu, L; Roumeliotis, TI; et al. (CELL PRESS, 2019-08-06)Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, ... -
Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ.
Pollard, DJ; Berger, CN; So, EC; Yu, L; Hadavizadeh, K; et al. (AMER SOC MICROBIOLOGY, 2018-04-10)Tyrosine phosphorylation is key for signal transduction from exogenous stimuli, including the defense against pathogens. Conversely, pathogens can subvert protein phosphorylation to control host immune responses and ... -
C9orf72 arginine-rich dipeptide proteins interact with ribosomal proteins in vivo to induce a toxic translational arrest that is rescued by eIF1A.
Moens, TG; Niccoli, T; Wilson, KM; Atilano, ML; Birsa, N; et al. (SPRINGER, 2019-03-01)A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is the most common genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense and antisense repeat-containing transcripts undergo ... -
Cell-type-specific visualisation and biochemical isolation of endogenous synaptic proteins in mice.
Zhu, F; Collins, MO; Harmse, J; Choudhary, JS; Grant, SGN; et al. (WILEY, 2020-02-01)In recent years, the remarkable molecular complexity of synapses has been revealed, with over 1,000 proteins identified in the synapse proteome. Although it is known that different receptors and other synaptic proteins are ... -
Characterization of proteome-size scaling by integrative omics reveals mechanisms of proliferation control in cancer.
Jones, I; Dent, L; Higo, T; Roumeliotis, T; Arias Garcia, M; et al. (AMER ASSOC ADVANCEMENT SCIENCE, 2023-01-25)Almost all living cells maintain size uniformity through successive divisions. Proteins that over and underscale with size can act as rheostats, which regulate cell cycle progression. Using a multiomic strategy, we leveraged ... -
Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells.
Bode, D; Yu, L; Tate, P; Pardo, M; Choudhary, J (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2016-03-01)Pluripotency and self-renewal, the defining properties of embryonic stem cells, are brought about by transcriptional programs involving an intricate network of transcription factors and chromatin remodeling complexes. The ... -
Citrobacter rodentium induces rapid and unique metabolic and inflammatory responses in mice suffering from severe disease.
Carson, D; Barry, R; Hopkins, EGD; Roumeliotis, TI; García-Weber, D; et al. (WILEY-HINDAWI, 2020-01-01)The mouse pathogen Citrobacter rodentium is used to model infections with enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC). Pathogenesis is commonly modelled in mice developing mild disease (e.g., ... -
Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo.
Berger, CN; Crepin, VF; Roumeliotis, TI; Wright, JC; Carson, D; et al. (CELL PRESS, 2017-11-07)The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against ingested pathogens. We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC ... -
Clustering of Tir during enteropathogenic E. coli infection triggers calcium influx-dependent pyroptosis in intestinal epithelial cells.
Zhong, Q; Roumeliotis, TI; Kozik, Z; Cepeda-Molero, M; Fernández, LÁ; et al. (PUBLIC LIBRARY SCIENCE, 2020-12-30)Clustering of the enteropathogenic Escherichia coli (EPEC) type III secretion system (T3SS) effector translocated intimin receptor (Tir) by intimin leads to actin polymerisation and pyroptotic cell death in macrophages. ... -
CRISPR activation screen in mice identifies novel membrane proteins enhancing pulmonary metastatic colonisation.
van der Weyden, L; Harle, V; Turner, G; Offord, V; Iyer, V; et al. (NATURE PORTFOLIO, 2021-03-23)Melanoma represents ~5% of all cutaneous malignancies, yet accounts for the majority of skin cancer deaths due to its propensity to metastasise. To develop new therapies, novel target molecules must to be identified and ...