Browsing ICR Divisions by author "Mansfeld, Joerg"
Now showing items 1-13 of 13
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A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Kirova, DG; Judasova, K; Vorhauser, J; Zerjatke, T; Leung, JK; et al. (CELL PRESS, 2022-07-25)Reactive oxygen species (ROS) at the right concentration promote cell proliferation in cell culture, stem cells, and model organisms. However, the mystery of how ROS signaling is coordinated with cell cycle progression and ... -
An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules.
Bakos, G; Yu, L; Gak, IA; Roumeliotis, TI; Liakopoulos, D; et al. (NATURE PUBLISHING GROUP, 2018-11-14)Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck ... -
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Liess, AKL; Kucerova, A; Schweimer, K; Yu, L; Roumeliotis, TI; et al. (CELL PRESS, 2019-08-06)Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, ... -
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.
Daniel, K; Icha, J; Horenburg, C; Müller, D; Norden, C; et al. (NATURE PUBLISHING GROUP, 2018-08-17)The conditional and reversible depletion of proteins by auxin-mediated degradation is a powerful tool to investigate protein functions in cells and whole organisms. However, its wider applications require fusing the ... -
Constitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus.
Grant, R; Abdelbaki, A; Bertoldi, A; Gavilan, MP; Mansfeld, J; et al. (ROYAL SOC, 2018-06-13)Aurora A kinase (AURKA) is a major regulator of mitosis and an important driver of cancer progression. The roles of AURKA outside of mitosis, and how these might contribute to cancer progression, are not well understood. ... -
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.
Beer, KB; Fazeli, G; Judasova, K; Irmisch, L; Causemann, J; et al. (NATURE PUBLISHING GROUP, 2019-08-02)Visualization of specific organelles in tissues over background fluorescence can be challenging, especially when reporters localize to multiple structures. Instead of trying to identify proteins enriched in specific ... -
Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S.
Liess, AKL; Kucerova, A; Schweimer, K; Schlesinger, D; Dybkov, O; et al. (AMER ASSOC ADVANCEMENT SCIENCE, 2020-10-20)At the heart of protein ubiquitination cascades, ubiquitin-conjugating enzymes (E2s) form reactive ubiquitin-thioester intermediates to enable efficient transfer of ubiquitin to cellular substrates. The precise regulation ... -
Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity.
Ahmed Alfar, E; Kirova, D; Konantz, J; Birke, S; Mansfeld, J; et al. (NATURE PORTFOLIO, 2017-06-26)The pancreatic beta-cells control glucose homeostasis by secreting insulin in response to nutrient intake. The number of beta-cells is under tight metabolic control, as this number increases with higher nutrient intake. ... -
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.
Barr, AR; Cooper, S; Heldt, FS; Butera, F; Stoy, H; et al. (NATURE PUBLISHING GROUP, 2017-03-20)Following DNA damage caused by exogenous sources, such as ionizing radiation, the tumour suppressor p53 mediates cell cycle arrest via expression of the CDK inhibitor, p21. However, the role of p21 in maintaining genomic ... -
Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.
Uvizl, A; Goswami, R; Gandhi, SD; Augsburg, M; Buchholz, F; et al. (ROYAL SOC CHEMISTRY, 2021-06-15)Intracellular delivery of cargo molecules such as membrane-impermeable proteins or drugs is crucial for cell treatment in biological and medical applications. Recently, microfluidic mechanoporation techniques have enabled ... -
PLK1 Activation in Late G2 Sets Up Commitment to Mitosis.
Gheghiani, L; Loew, D; Lombard, B; Mansfeld, J; Gavet, O (CELL PRESS, 2017-06-06)Commitment to mitosis must be tightly coordinated with DNA replication to preserve genome integrity. While we have previously established that the timely activation of CyclinB1-Cdk1 in late G2 triggers mitotic entry, the ... -
Quantitative Cell Cycle Analysis Based on an Endogenous All-in-One Reporter for Cell Tracking and Classification.
Zerjatke, T; Gak, IA; Kirova, D; Fuhrmann, M; Daniel, K; et al. (CELL PRESS, 2017-05-30)Cell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in ... -
ROS Dynamics Delineate Functional States of Hippocampal Neural Stem Cells and Link to Their Activity-Dependent Exit from Quiescence.
Adusumilli, VS; Walker, TL; Overall, RW; Klatt, GM; Zeidan, SA; et al. (CELL PRESS, 2021-02-04)Cellular redox states regulate the balance between stem cell maintenance and activation. Increased levels of intracellular reactive oxygen species (ROS) are linked to proliferation and lineage specification. In contrast ...