dc.contributor.author | Mouratidis, PXE | |
dc.contributor.author | Rivens, I | |
dc.contributor.author | Civale, J | |
dc.contributor.author | Symonds-Tayler, R | |
dc.contributor.author | Ter Haar, G | |
dc.date.accessioned | 2019-02-15T16:02:13Z | |
dc.date.issued | 2019-01-01 | |
dc.identifier.citation | International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 2019, 36 (1), pp. 229 - 243 | |
dc.identifier.issn | 0265-6736 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3053 | |
dc.identifier.eissn | 1464-5157 | |
dc.identifier.doi | 10.1080/02656736.2018.1558289 | |
dc.description.abstract | AIM: Thermal isoeffective dose (TID) has not been convincingly validated for application to predict biological effects from rapid thermal ablation (e.g., using >55 °C). This study compares the classical method of quantifying TID (derived from hyperthermia data) with a temperature-adjusted method based on the Arrhenius model for predicting cell survival in vitro, after either 'rapid' ablative or 'slow' hyperthermic exposures. METHODS: MTT assay viability data was obtained from two human colon cancer cell lines, (HCT116, HT29), subjected to a range of TIDs (120-720 CEM43) using a thermal cycler for hyperthermic (>2 minutes, <50 °C) treatments, or a novel pre-heated water bath based technique for ablative exposures (<10 seconds, >55 °C). TID was initially estimated using a constant RCEM>43°C=0.5, and subsequently using RCEM(T), derived from temperature dependent cell survival (injury rate) Arrhenius analysis. RESULTS: 'Slow' and 'rapid' exposures resulted in cell survival and significant regrowth (both cell lines) 10 days post-treatment for 240 CEM43 (RCEM>43°C=0.5), while 340-550 CEM43 (RCEM>43°C =0.5) delivered using 'rapid' exposures showed 12 ± 6% viability and 'slow' exposures resulted in undetectable viability. Arrhenius analysis of experimental data (activation energy ΔE = 5.78 ± 0.04 × 105 J mole-1, frequency factor A = 3.27 ± 11 × 1091 sec-1) yielded RCEM=0.42 * e0.0041*T which better-predicted cell survival than using R CEM> 43°C=0.5. CONCLUSIONS: TID calculated using an RCEM(T) informed by Arrhenius kinetic parameters provided a more consistent, heating strategy independent, predictor of cell viability, improving dosimetry of ablative thermal exposures. Cell viability was only undetectable above 305 ± 10 CEM43 using this revised measure. | |
dc.format | Print-Electronic | |
dc.format.extent | 229 - 243 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | TAYLOR & FRANCIS LTD | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Humans | |
dc.subject | Hyperthermia, Induced | |
dc.subject | Heating | |
dc.subject | Cell Death | |
dc.title | 'Relationship between thermal dose and cell death for "rapid" ablative and "slow" hyperthermic heating'. | |
dc.type | Journal Article | |
rioxxterms.versionofrecord | 10.1080/02656736.2018.1558289 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2019-01-31 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Imaging for Radiotherapy Adaptation | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Therapeutic Ultrasound | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Imaging for Radiotherapy Adaptation | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Therapeutic Ultrasound | |
pubs.publication-status | Published | |
pubs.volume | 36 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Imaging for Radiotherapy Adaptation | |
icr.researchteam | Therapeutic Ultrasound | |
dc.contributor.icrauthor | Mouratidis, Petros | |
dc.contributor.icrauthor | Rivens, Ian | |
dc.contributor.icrauthor | Civale, John | |
dc.contributor.icrauthor | Ter Haar, Gail | |