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dc.contributor.authorFassan, Men_US
dc.contributor.authorVianello, Len_US
dc.contributor.authorSacchi, Den_US
dc.contributor.authorFanelli, GNen_US
dc.contributor.authorMunari, Gen_US
dc.contributor.authorScarpa, Men_US
dc.contributor.authorCappellesso, Ren_US
dc.contributor.authorLoupakis, Fen_US
dc.contributor.authorLanza, Cen_US
dc.contributor.authorSalmaso, Ren_US
dc.contributor.authorMescoli, Cen_US
dc.contributor.authorValeri, Nen_US
dc.contributor.authorAgostini, Men_US
dc.contributor.authorD'Angelo, Een_US
dc.contributor.authorLonardi, Sen_US
dc.contributor.authorPucciarelli, Sen_US
dc.contributor.authorVeronese, Nen_US
dc.contributor.authorLuchini, Cen_US
dc.contributor.authorRugge, Men_US
dc.coverage.spatialEnglanden_US
dc.date.accessioned2019-02-20T07:44:51Z
dc.date.issued2018en_US
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/30202242en_US
dc.identifier634en_US
dc.identifier.citationCancer Cell Int, 2018, 18 pp. 131 - ?en_US
dc.identifier.issn1475-2867en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3058
dc.identifier.doi10.1186/s12935-018-0634-8en_US
dc.description.abstractBackground: No data is available on the molecular background of the extra-nodal extension (ENE) of lymph node metastasis (LN) in colorectal cancer (CRC). Methods: A series of 22 ENE-positive CRCs was considered and three samples per case were selected (the primary CRC, an ENE-negative and an ENE-positive metastatic LN). Samples (n = 66) were analysed by immunohistochemistry for PD-L1, CD4, CD8, CD68 and CD80. Fifteen out of twenty-two cases were further profiled through a hotspot multigene mutational custom panel, including 164 hotspot regions of AKT1, APC, BRAF, CTNNB1, KIT, KRAS, NRAS, PDGFRA, PIK3CA, PTEN and TP53 genes. Results: A significantly higher percentage of CD4-, CD8- and CD68-positive cells was observed at the invasive front of both CRCs and in ENE in contrast with what observed at the core of both CRCs and their matched nodal metastases. ENE was also characterized by a significantly higher number of CD80-positive cells. No significant difference was observed in PD-L1 distribution among the different specimens. Fourteen out of 15 CRCs (93%) showed at least a driver mutation. The most frequently mutated gene was TP53 (n = 8 tumors), followed by APC (n = 6), BRAF (n = 4), KRAS, NRAS and PIK3CA (n = 2). In 11 out of 15 CRCs (73%) the mutational profiling of the primary tumor was consistent with what obtained from the two matched LNs. Conclusions: A heterogeneous intratumor immune-microenvironment has been observed in ENE-positive CRCs, which are characterized by an increased leukocytic infiltration at the ENE invasive front.en_US
dc.format.extent131 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectBiomarkersen_US
dc.subjectColorectal canceren_US
dc.subjectExtranodal extensionen_US
dc.subjectMetastasisen_US
dc.titleAssessment of intratumor immune-microenvironment in colorectal cancers with extranodal extension of nodal metastases.en_US
dc.typeJournal Article
dcterms.dateAccepted2018-09-03en_US
rioxxterms.versionofrecord10.1186/s12935-018-0634-8en_US
rioxxterms.licenseref.startdate2018en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfCancer Cell Inten_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Gastrointestinal Cancer Biology and Genomics
pubs.publication-statusPublished onlineen_US
pubs.volume18en_US
pubs.embargo.termsNot knownen_US
icr.researchteamGastrointestinal Cancer Biology and Genomicsen_US
dc.contributor.icrauthorValeri, Nicolaen_US


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