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dc.contributor.authorBock, LJen_US
dc.contributor.authorPagliuca, Cen_US
dc.contributor.authorKobayashi, Nen_US
dc.contributor.authorGrove, RAen_US
dc.contributor.authorOku, Yen_US
dc.contributor.authorShrestha, Ken_US
dc.contributor.authorAlfieri, Cen_US
dc.contributor.authorGolfieri, Cen_US
dc.contributor.authorOldani, Aen_US
dc.contributor.authorDal Maschio, Men_US
dc.contributor.authorBermejo, Ren_US
dc.contributor.authorHazbun, TRen_US
dc.contributor.authorTanaka, TUen_US
dc.contributor.authorDe Wulf, Pen_US
dc.date.accessioned2019-11-15T14:42:24Z
dc.date.issued2012-05-06en_US
dc.identifier.citationNature cell biology, 2012, 14 (6), pp. 614 - 624en_US
dc.identifier.issn1465-7392en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3418
dc.identifier.eissn1476-4679en_US
dc.identifier.doi10.1038/ncb2495en_US
dc.description.abstractKinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B(Ipl1) and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.en_US
dc.formatElectronicen_US
dc.format.extent614 - 624en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
dc.subjectChromosomes, Fungalen_US
dc.subjectKinetochoresen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectProtein-Serine-Threonine Kinasesen_US
dc.subjectCell Cycle Proteinsen_US
dc.subjectMicrotubule-Associated Proteinsen_US
dc.subjectSaccharomyces cerevisiae Proteinsen_US
dc.subjectAnaphaseen_US
dc.subjectProtein Bindingen_US
dc.subjectPhosphorylationen_US
dc.subjectSpindle Apparatusen_US
dc.titleCnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.en_US
dc.typeJournal Article
dcterms.dateAccepted2012-04-02en_US
rioxxterms.versionofrecord10.1038/ncb2495en_US
rioxxterms.licenseref.startdate2012-05-06en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfNature cell biologyen_US
pubs.issue6en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Molecular mechanisms of cell cycle regulation
pubs.publication-statusPublisheden_US
pubs.volume14en_US
pubs.embargo.termsNot knownen_US
icr.researchteamMolecular mechanisms of cell cycle regulationen_US
dc.contributor.icrauthorAlfieri, Claudioen_US


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