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Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.

dc.contributor.authorBock, LJ
dc.contributor.authorPagliuca, C
dc.contributor.authorKobayashi, N
dc.contributor.authorGrove, RA
dc.contributor.authorOku, Y
dc.contributor.authorShrestha, K
dc.contributor.authorAlfieri, C
dc.contributor.authorGolfieri, C
dc.contributor.authorOldani, A
dc.contributor.authorDal Maschio, M
dc.contributor.authorBermejo, R
dc.contributor.authorHazbun, TR
dc.contributor.authorTanaka, TU
dc.contributor.authorDe Wulf, P
dc.date.accessioned2019-11-15T14:42:24Z
dc.date.issued2012-05-06
dc.identifier.citationNature cell biology, 2012, 14 (6), pp. 614 - 624
dc.identifier.issn1465-7392
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/3418
dc.identifier.eissn1476-4679
dc.identifier.doi10.1038/ncb2495
dc.description.abstractKinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B(Ipl1) and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.
dc.formatElectronic
dc.format.extent614 - 624
dc.languageeng
dc.language.isoeng
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved
dc.subjectChromosomes, Fungal
dc.subjectKinetochores
dc.subjectSaccharomyces cerevisiae
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectCell Cycle Proteins
dc.subjectMicrotubule-Associated Proteins
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectAnaphase
dc.subjectProtein Binding
dc.subjectPhosphorylation
dc.subjectSpindle Apparatus
dc.titleCnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.
dc.typeJournal Article
dcterms.dateAccepted2012-04-02
rioxxterms.versionofrecord10.1038/ncb2495
rioxxterms.licenseref.urihttps://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2012-05-06
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNature cell biology
pubs.issue6
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Molecular mechanisms of cell cycle regulation
pubs.publication-statusPublished
pubs.volume14
pubs.embargo.termsNot known
icr.researchteamMolecular mechanisms of cell cycle regulation
dc.contributor.icrauthorAlfieri, Claudio


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