Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.
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Date
2012-05-06ICR Author
Author
Bock, LJ
Pagliuca, C
Kobayashi, N
Grove, RA
Oku, Y
Shrestha, K
Alfieri, C
Golfieri, C
Oldani, A
Dal Maschio, M
Bermejo, R
Hazbun, TR
Tanaka, TU
De Wulf, P
Type
Journal Article
Metadata
Show full item recordAbstract
Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B(Ipl1) and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.
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Subject
Chromosomes, Fungal
Kinetochores
Saccharomyces cerevisiae
Protein-Serine-Threonine Kinases
Cell Cycle Proteins
Microtubule-Associated Proteins
Saccharomyces cerevisiae Proteins
Anaphase
Protein Binding
Phosphorylation
Spindle Apparatus
Research team
Molecular mechanisms of cell cycle regulation
Language
eng
Date accepted
2012-04-02
License start date
2012-05-06
Citation
Nature cell biology, 2012, 14 (6), pp. 614 - 624
Publisher
NATURE PUBLISHING GROUP
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