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dc.contributor.authorRata, M
dc.contributor.authorGiles, SL
dc.contributor.authordeSouza, NM
dc.contributor.authorLeach, MO
dc.contributor.authorPayne, GS
dc.date.accessioned2016-12-23T11:04:11Z
dc.date.issued2014-02-01
dc.identifier.citationNMR in biomedicine, 2014, 27 (2), pp. 158 - 162
dc.identifier.issn0952-3480
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/353
dc.identifier.eissn1099-1492
dc.identifier.doi10.1002/nbm.3047
dc.description.abstract31P magnetic resonance spectroscopy (31P MRS) can measure intracellular pH (pHi) using the chemical shift difference between pH-dependent inorganic phosphate (Pi) and a pH-independent reference peak. This study compared three different frequency reference peaks [phosphocreatine (PCr), α resonance of adenosine triphosphate (αATP) and water (using 1H MRS)] in a cohort of 10 volunteers and eight patients with non-Hodgkin's lymphoma (NHL). Well-resolved chemical shift imaging (CSI) spectra were acquired on a 1.5T scanner for muscle, liver and tumour. The pH was calculated for all volunteers and patients using the available methods. The consistency of the resulting pH was evaluated. The direct Pi–PCr method was best for those spectra with a very well-defined PCr, such as muscle (pH=7.05 ± 0.02). In liver, the Pi–αATP method gave more consistent results (pH=7.30 ± 0.06) than the calibrated water-based method (pH=7.27 ± 0.11). In NHL nodes, the measured pH using the Pi–αATP method was 7.25 ± 0.12. Given that the measured range includes some biological variation in individual patients, treatment-related changes of the order of 0.1 pH units should be detectable.
dc.formatPrint
dc.format.extent158 - 162
dc.languageeng
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectHumans
dc.subjectLymphoma, Non-Hodgkin
dc.subjectPhosphates
dc.subjectPhosphorus Isotopes
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectSensitivity and Specificity
dc.subjectReproducibility of Results
dc.subjectHydrogen-Ion Concentration
dc.subjectAlgorithms
dc.subjectReference Values
dc.subjectAdult
dc.subjectAged
dc.subjectMiddle Aged
dc.subjectFemale
dc.subjectMale
dc.titleComparison of three reference methods for the measurement of intracellular pH using 31P MRS in healthy volunteers and patients with lymphoma.
dc.typeJournal Article
rioxxterms.versionofrecord10.1002/nbm.3047
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2014-02
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfNMR in biomedicine
pubs.issue2
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance
pubs.publication-statusPublished
pubs.volume27
pubs.embargo.termsNot known
icr.researchteamMagnetic Resonance
dc.contributor.icrauthordeSouza, Nandita
dc.contributor.icrauthorLeach, Martin


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