dc.contributor.author | Visone, R | |
dc.contributor.author | Bacalini, MG | |
dc.contributor.author | Di Franco, S | |
dc.contributor.author | Ferracin, M | |
dc.contributor.author | Colorito, ML | |
dc.contributor.author | Pagotto, S | |
dc.contributor.author | Laprovitera, N | |
dc.contributor.author | Licastro, D | |
dc.contributor.author | Di Marco, M | |
dc.contributor.author | Scavo, E | |
dc.contributor.author | Bassi, C | |
dc.contributor.author | Saccenti, E | |
dc.contributor.author | Nicotra, A | |
dc.contributor.author | Grzes, M | |
dc.contributor.author | Garagnani, P | |
dc.contributor.author | De Laurenzi, V | |
dc.contributor.author | Valeri, N | |
dc.contributor.author | Mariani-Costantini, R | |
dc.contributor.author | Negrini, M | |
dc.contributor.author | Stassi, G | |
dc.contributor.author | Veronese, A | |
dc.date.accessioned | 2020-06-08T14:41:01Z | |
dc.date.issued | 2019-05-01 | |
dc.identifier.citation | Epigenomics, 2019, 11 (6), pp. 587 - 604 | |
dc.identifier.issn | 1750-1911 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3703 | |
dc.identifier.eissn | 1750-192X | |
dc.identifier.doi | 10.2217/epi-2018-0153 | |
dc.description.abstract | Aim: To investigate the genome-wide methylation of genetically characterized colorectal cancer stem cell (CR-CSC) lines. Materials & methods: Eight CR-CSC lines were isolated from primary colorectal cancer (CRC) tissues, cultured and characterized for aneuploidy, mutational status of CRC-related genes and microsatellite instability (MSI). Genome-wide DNA methylation was assessed by MethylationEPIC microarray. Results: We describe a distinctive methylation pattern that is maintained following in vivo passages in immune-compromised mice. We identified an epigenetic CR-CSC signature associated with MSI. We noticed that the preponderance of the differentially methylated positions do not reside at CpG islands, but spread to shelf and open sea regions. Conclusion: Given that CRCs with MSI-high status have a lower metastatic potential, the identification of a MSI-related methylation signature could provide new insights and possible targets into metastatic CRC. | |
dc.format | Print-Electronic | |
dc.format.extent | 587 - 604 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | FUTURE MEDICINE LTD | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Animals | |
dc.subject | Humans | |
dc.subject | Mice | |
dc.subject | Colonic Neoplasms | |
dc.subject | DNA Methylation | |
dc.subject | Epigenesis, Genetic | |
dc.subject | CpG Islands | |
dc.subject | Drug Resistance, Neoplasm | |
dc.subject | Microsatellite Instability | |
dc.subject | Neoplastic Stem Cells | |
dc.subject | Heterografts | |
dc.title | DNA methylation of shelf, shore and open sea CpG positions distinguish high microsatellite instability from low or stable microsatellite status colon cancer stem cells. | |
dc.type | Journal Article | |
rioxxterms.versionofrecord | 10.2217/epi-2018-0153 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2019-05-08 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Epigenomics | |
pubs.issue | 6 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Gastrointestinal Cancer Biology and Genomics | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Gastrointestinal Cancer Biology and Genomics | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Gastrointestinal Cancer Biology and Genomics | |
dc.contributor.icrauthor | Valeri, Nicola | |