dc.contributor.author | Piccirillo, SGM | |
dc.contributor.author | Colman, S | |
dc.contributor.author | Potter, NE | |
dc.contributor.author | van Delft, FW | |
dc.contributor.author | Lillis, S | |
dc.contributor.author | Carnicer, M-J | |
dc.contributor.author | Kearney, L | |
dc.contributor.author | Watts, C | |
dc.contributor.author | Greaves, M | |
dc.date.accessioned | 2020-08-05T13:56:34Z | |
dc.date.issued | 2015-01-13 | |
dc.identifier.citation | Stem cell reports, 2015, 4 (1), pp. 7 - 15 | |
dc.identifier.issn | 2213-6711 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/3908 | |
dc.identifier.eissn | 2213-6711 | |
dc.identifier.doi | 10.1016/j.stemcr.2014.11.003 | |
dc.description.abstract | Glioblastoma (GBM) is a lethal malignancy whose clinical intransigence has been linked to extensive intraclonal genetic and phenotypic diversity and the common emergence of therapeutic resistance. This interpretation embodies the implicit assumption that cancer stem cells or tumor-propagating cells are themselves genetically and functionally diverse. To test this, we screened primary GBM tumors by SNP array to identify copy number alterations (a minimum of three) that could be visualized in single cells by multicolor fluorescence in situ hybridization. Interrogation of neurosphere-derived cells (from four patients) and cells derived from secondary transplants of these same cells in NOD-SCID mice allowed us to infer the clonal and phylogenetic architectures. Whole-exome sequencing and single-cell genetic analysis in one case revealed a more complex clonal structure. This proof-of-principle experiment revealed that subclones in each GBM had variable regenerative or stem cell activity, and highlighted genetic alterations associated with more competitive propagating activity in vivo. | |
dc.format | Print-Electronic | |
dc.format.extent | 7 - 15 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | CELL PRESS | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | |
dc.subject | Cell Line, Tumor | |
dc.subject | Animals | |
dc.subject | Humans | |
dc.subject | Mice | |
dc.subject | Glioblastoma | |
dc.subject | Brain Neoplasms | |
dc.subject | Disease Progression | |
dc.subject | In Situ Hybridization, Fluorescence | |
dc.subject | Genomics | |
dc.subject | Phenotype | |
dc.subject | Polymorphism, Single Nucleotide | |
dc.subject | Neoplastic Stem Cells | |
dc.subject | Genetic Variation | |
dc.subject | Genome-Wide Association Study | |
dc.subject | DNA Copy Number Variations | |
dc.subject | Single-Cell Analysis | |
dc.subject | High-Throughput Nucleotide Sequencing | |
dc.subject | Heterografts | |
dc.title | Genetic and functional diversity of propagating cells in glioblastoma. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2014-11-18 | |
rioxxterms.versionofrecord | 10.1016/j.stemcr.2014.11.003 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | |
rioxxterms.licenseref.startdate | 2015-01 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Stem cell reports | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Biology of Childhood Leukaemia | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Biology of Childhood Leukaemia | |
pubs.publication-status | Published | |
pubs.volume | 4 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Biology of Childhood Leukaemia | |
dc.contributor.icrauthor | Greaves, Melvyn | |