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dc.contributor.authorEvans, IM
dc.contributor.authorKennedy, SA
dc.contributor.authorPaliashvili, K
dc.contributor.authorSantra, T
dc.contributor.authorYamaji, M
dc.contributor.authorLovering, RC
dc.contributor.authorBritton, G
dc.contributor.authorFrankel, P
dc.contributor.authorKolch, W
dc.contributor.authorZachary, IC
dc.date.accessioned2020-11-23T12:08:53Z
dc.date.issued2017-02
dc.identifier.citationMolecular & cellular proteomics : MCP, 2017, 16 (2), pp. 168 - 180
dc.identifier.issn1535-9476
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4236
dc.identifier.eissn1535-9484
dc.identifier.doi10.1074/mcp.m116.064428
dc.description.abstractp130Cas is a polyvalent adapter protein essential for cardiovascular development, and with a key role in cell movement. In order to identify the pathways by which p130Cas exerts its biological functions in endothelial cells we mapped the p130Cas interactome and its dynamic changes in response to VEGF using high-resolution mass spectrometry and reconstruction of protein interaction (PPI) networks with the aid of multiple PPI databases. VEGF enriched the p130Cas interactome in proteins involved in actin cytoskeletal dynamics and cell movement, including actin-binding proteins, small GTPases and regulators or binders of GTPases. Detailed studies showed that p130Cas association of the GTPase-binding scaffold protein, IQGAP1, plays a key role in VEGF chemotactic signaling, endothelial polarization, VEGF-induced cell migration, and endothelial tube formation. These findings indicate a cardinal role for assembly of the p130Cas interactome in mediating the cell migratory response to VEGF in angiogenesis, and provide a basis for further studies of p130Cas in cell movement.
dc.formatPrint-Electronic
dc.format.extent168 - 180
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectHumans
dc.subjectVascular Endothelial Growth Factor A
dc.subjectProteomics
dc.subjectSignal Transduction
dc.subjectChemotaxis
dc.subjectNeovascularization, Physiologic
dc.subjectDatabases, Protein
dc.subjectCrk-Associated Substrate Protein
dc.subjectMass Spectrometry
dc.subjectHuman Umbilical Vein Endothelial Cells
dc.subjectProtein Interaction Maps
dc.titleVascular Endothelial Growth Factor (VEGF) Promotes Assembly of the p130Cas Interactome to Drive Endothelial Chemotactic Signaling and Angiogenesis.
dc.typeJournal Article
dcterms.dateAccepted2016-12-15
rioxxterms.versionofrecord10.1074/mcp.m116.064428
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2017-02
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfMolecular & cellular proteomics : MCP
pubs.issue2
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell
pubs.publication-statusPublished
pubs.volume16
pubs.embargo.termsNot known
icr.researchteamCancer Stem Cellen_US
dc.contributor.icrauthorEvans, Ianen


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