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dc.contributor.authorEvans, IMen_US
dc.contributor.authorKennedy, SAen_US
dc.contributor.authorPaliashvili, Ken_US
dc.contributor.authorSantra, Ten_US
dc.contributor.authorYamaji, Men_US
dc.contributor.authorLovering, RCen_US
dc.contributor.authorBritton, Gen_US
dc.contributor.authorFrankel, Pen_US
dc.contributor.authorKolch, Wen_US
dc.contributor.authorZachary, ICen_US
dc.date.accessioned2020-11-23T12:08:53Z
dc.date.issued2017-02en_US
dc.identifier.citationMolecular & cellular proteomics : MCP, 2017, 16 (2), pp. 168 - 180en_US
dc.identifier.issn1535-9476en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4236
dc.identifier.eissn1535-9484en_US
dc.identifier.doi10.1074/mcp.m116.064428en_US
dc.description.abstractp130Cas is a polyvalent adapter protein essential for cardiovascular development, and with a key role in cell movement. In order to identify the pathways by which p130Cas exerts its biological functions in endothelial cells we mapped the p130Cas interactome and its dynamic changes in response to VEGF using high-resolution mass spectrometry and reconstruction of protein interaction (PPI) networks with the aid of multiple PPI databases. VEGF enriched the p130Cas interactome in proteins involved in actin cytoskeletal dynamics and cell movement, including actin-binding proteins, small GTPases and regulators or binders of GTPases. Detailed studies showed that p130Cas association of the GTPase-binding scaffold protein, IQGAP1, plays a key role in VEGF chemotactic signaling, endothelial polarization, VEGF-induced cell migration, and endothelial tube formation. These findings indicate a cardinal role for assembly of the p130Cas interactome in mediating the cell migratory response to VEGF in angiogenesis, and provide a basis for further studies of p130Cas in cell movement.en_US
dc.formatPrint-Electronicen_US
dc.format.extent168 - 180en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectHumansen_US
dc.subjectVascular Endothelial Growth Factor Aen_US
dc.subjectProteomicsen_US
dc.subjectSignal Transductionen_US
dc.subjectChemotaxisen_US
dc.subjectNeovascularization, Physiologicen_US
dc.subjectDatabases, Proteinen_US
dc.subjectCrk-Associated Substrate Proteinen_US
dc.subjectMass Spectrometryen_US
dc.subjectHuman Umbilical Vein Endothelial Cellsen_US
dc.subjectProtein Interaction Mapsen_US
dc.titleVascular Endothelial Growth Factor (VEGF) Promotes Assembly of the p130Cas Interactome to Drive Endothelial Chemotactic Signaling and Angiogenesis.en_US
dc.typeJournal Article
dcterms.dateAccepted2016-12-15en_US
rioxxterms.versionofrecord10.1074/mcp.m116.064428en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2017-02en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMolecular & cellular proteomics : MCPen_US
pubs.issue2en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell
pubs.publication-statusPublisheden_US
pubs.volume16en_US
pubs.embargo.termsNot knownen_US
icr.researchteamCancer Stem Cellen_US
dc.contributor.icrauthorEvans, Ianen_US


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