Show simple item record

dc.contributor.authorBery, Nen_US
dc.contributor.authorRabbitts, THen_US
dc.date.accessioned2021-01-13T12:32:48Z
dc.date.issued2019-06en_US
dc.identifier.citationCurrent protocols in cell biology, 2019, 83 (1), pp. e83 - ?en_US
dc.identifier.issn1934-2500en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4280
dc.identifier.eissn1934-2616en_US
dc.identifier.doi10.1002/cpcb.83en_US
dc.description.abstractProtein-protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challenging to convert to therapeutics. In the last decade, much progress has been made in the inhibition of PPIs involved in diseases, but many remain difficult such as RAS-effector interactions in cancers. We describe here a protocol for using Bioluminescence Resonance Energy Transfer 2 (BRET2)-based RAS biosensors to detect and characterize RAS PPI inhibition by macromolecules and small molecules. This method could be extended to any other small GTPases or any other PPIs of interest. © 2019 by John Wiley & Sons, Inc.en_US
dc.formatPrint-Electronicen_US
dc.format.extente83 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
dc.subjectHumansen_US
dc.subjectras Proteinsen_US
dc.subjectImmunoblottingen_US
dc.subjectLuminescent Measurementsen_US
dc.subjectProtein Engineeringen_US
dc.subjectBiosensing Techniquesen_US
dc.subjectEnergy Transferen_US
dc.subjectHEK293 Cellsen_US
dc.titleBioluminescence Resonance Energy Transfer 2 (BRET2)-Based RAS Biosensors to Characterize RAS Inhibitors.en_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1002/cpcb.83en_US
rioxxterms.licenseref.startdate2019-06en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfCurrent protocols in cell biologyen_US
pubs.issue1en_US
pubs.notesNo embargoen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Chromosomal Translocations and Intracellular Antibody Therapeutics
pubs.publication-statusPublisheden_US
pubs.volume83en_US
pubs.embargo.termsNo embargoen_US
icr.researchteamChromosomal Translocations and Intracellular Antibody Therapeuticsen_US
dc.contributor.icrauthorRabbitts, Terenceen_US


Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record