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Bioluminescence Resonance Energy Transfer 2 (BRET2)-Based RAS Biosensors to Characterize RAS Inhibitors.

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Publication Date
2019-06
ICR Author
Rabbitts, Terence
Author
Bery, N
Rabbitts, TH
Type
Journal Article
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Abstract
Protein-protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challenging to convert to therapeutics. In the last decade, much progress has been made in the inhibition of PPIs involved in diseases, but many remain difficult such as RAS-effector interactions in cancers. We describe here a protocol for using Bioluminescence Resonance Energy Transfer 2 (BRET2)-based RAS biosensors to detect and characterize RAS PPI inhibition by macromolecules and small molecules. This method could be extended to any other small GTPases or any other PPIs of interest. © 2019 by John Wiley & Sons, Inc.
URL
https://repository.icr.ac.uk/handle/internal/4280
Collections
  • Cancer Therapeutics
Licenseref URL
http://www.rioxx.net/licenses/all-rights-reserved
Version of record
10.1002/cpcb.83
Subject
Humans
ras Proteins
Immunoblotting
Luminescent Measurements
Protein Engineering
Biosensing Techniques
Energy Transfer
HEK293 Cells
Research team
Chromosomal Translocations and Intracellular Antibody Therapeutics
Language
eng
License start date
2019-06
Citation
Current protocols in cell biology, 2019, 83 (1), pp. e83 - ?

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