dc.contributor.author | Chung, Y-L | |
dc.contributor.author | Leach, MO | |
dc.contributor.author | Eykyn, TR | |
dc.date.accessioned | 2017-03-01T13:12:44Z | |
dc.date.issued | 2017-01-01 | |
dc.identifier.citation | Methods in enzymology, 2017, 588 pp. 133 - 153 | |
dc.identifier.issn | 0076-6879 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/456 | |
dc.identifier.eissn | 1557-7988 | |
dc.identifier.doi | 10.1016/bs.mie.2016.09.078 | |
dc.description.abstract | Cancer cells undergoing starvation- and treatment-induced autophagy were found to exhibit reduced intracellular lactate, reduced rates of steady-state lactate excretion and reduced real-time pyruvate-lactate exchange rates, indicating that glycolytic metabolism was altered in autophagic cells. In this chapter, we describe the technical details of the use of 1H-magnetic resonance spectroscopy (MRS) to measure endogenous cellular concentrations of lactate and glucose in autophagic cells and tissues, how to measure the rate of steady-state lactate excretion and glucose uptake by 1H-MRS in autophagic cells, and details of the real-time measurement of [1-13C] pyruvate to lactate exchange in autophagic cells by 13C-MRS-DNP (dynamic nuclear polarization). | |
dc.format | Print-Electronic | |
dc.format.extent | 133 - 153 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | ELSEVIER ACADEMIC PRESS INC | |
dc.rights.uri | https://www.rioxx.net/licenses/all-rights-reserved | |
dc.subject | Animals | |
dc.subject | Humans | |
dc.subject | Carbon Isotopes | |
dc.subject | Lactic Acid | |
dc.subject | Pyruvic Acid | |
dc.subject | Glucose | |
dc.subject | Magnetic Resonance Spectroscopy | |
dc.subject | Glycolysis | |
dc.subject | Autophagy | |
dc.title | Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy. | |
dc.type | Journal Article | |
rioxxterms.versionofrecord | 10.1016/bs.mie.2016.09.078 | |
rioxxterms.licenseref.uri | https://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2017-01 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Methods in enzymology | |
pubs.notes | Indefinite | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance | |
pubs.publication-status | Published | |
pubs.volume | 588 | |
pubs.embargo.terms | Indefinite | |
icr.researchteam | Magnetic Resonance | |
dc.contributor.icrauthor | Chung, Yuen-Li | |
dc.contributor.icrauthor | Leach, Martin | |