dc.contributor.author | Gribben, C | |
dc.contributor.author | Lambert, C | |
dc.contributor.author | Messal, HA | |
dc.contributor.author | Hubber, E-L | |
dc.contributor.author | Rackham, C | |
dc.contributor.author | Evans, I | |
dc.contributor.author | Heimberg, H | |
dc.contributor.author | Jones, P | |
dc.contributor.author | Sancho, R | |
dc.contributor.author | Behrens, A | |
dc.date.accessioned | 2021-09-06T10:13:09Z | |
dc.date.available | 2021-09-06T10:13:09Z | |
dc.date.issued | 2021-11-04 | |
dc.identifier.citation | Cell Stem Cell, 2021 | |
dc.identifier.issn | 1934-5909 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4791 | |
dc.identifier.doi | 10.1016/j.stem.2021.08.003 | |
dc.description.abstract | Ductal cells have been proposed as a source of adult β cell neogenesis, but this has remained controversial. By combining lineage tracing, 3D imaging, and single-cell RNA sequencing (scRNA-seq) approaches, we show that ductal cells contribute to the β cell population over time. Lineage tracing using the Neurogenin3 (Ngn3)-CreERT line identified ductal cells expressing the endocrine master transcription factor Ngn3 that were positive for the δ cell marker somatostatin and occasionally co-expressed insulin. The number of hormone-expressing ductal cells was increased in Akita+/- diabetic mice, and ngn3 heterozygosity accelerated diabetes onset. scRNA-seq of Ngn3 lineage-traced islet cells indicated that duct-derived somatostatin-expressing cells, some of which retained expression of ductal markers, gave rise to β cells. This study identified Ngn3-expressing ductal cells as a source of adult β cell neogenesis in homeostasis and diabetes, suggesting that this mechanism, in addition to β cell proliferation, maintains the adult islet β cell population. | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | CELL PRESS | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.title | Ductal Ngn3-expressing progenitors contribute to adult β cell neogenesis in the pancreas. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2021-08-05 | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1016/j.stem.2021.08.003 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Cell Stem Cell | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell | |
pubs.publication-status | Published | |
pubs.embargo.terms | Not known | |
icr.researchteam | Cancer Stem Cell | |
icr.researchteam | Cancer Stem Cell | |
dc.contributor.icrauthor | Evans, Ian | |
dc.contributor.icrauthor | Behrens, Axel | |