Show simple item record

dc.contributor.authorNuta, O
dc.contributor.authorSomaiah, N
dc.contributor.authorBoyle, S
dc.contributor.authorChua, MLK
dc.contributor.authorGothard, L
dc.contributor.authorYarnold, J
dc.contributor.authorRothkamm, K
dc.contributor.authorHerskind, C
dc.date.accessioned2017-03-09T14:21:33Z
dc.date.issued2016-05
dc.identifier.citationCancer letters, 2016, 374 (2), pp. 324 - 330
dc.identifier.issn0304-3835
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/480
dc.identifier.eissn1872-7980
dc.identifier.doi10.1016/j.canlet.2016.02.036
dc.description.abstractLate normal tissue toxicity varies widely between patients and limits breast radiotherapy dose. Here we aimed to determine its relationship to DNA damage responses of fibroblast cultures from individual patients. Thirty-five breast cancer patients, with minimal or marked breast changes after breast-conserving therapy consented to receive a 4 Gy test irradiation to a small skin field of the left buttock and have punch biopsies taken from irradiated and unirradiated skin. Early-passage fibroblast cultures were established by outgrowth and irradiated in vitro with 0 or 4 Gy. 53BP1 foci, p53 and p21/CDKN1A were detected by immunofluorescence microscopy. Residual 53BP1 foci counts 24 h after in vitro irradiation were significantly higher in fibroblasts from RT-sensitive versus RT-resistant patients. Furthermore, significantly larger fractions of p53- but not p21/CDKN1A-positive fibroblasts were found in cultures from RT-sensitive patients without in vitro irradiation, and 2 h and 6 d post-irradiation. Exploratory analysis showed a stronger p53 response 2 h after irradiation of fibroblasts established from patients with severe reaction. These results associate the radiation response of fibroblasts with late reaction of the breast after RT and suggest a correlation with severity.
dc.formatPrint-Electronic
dc.format.extent324 - 330
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectBreast Neoplasms
dc.subjectRadiation Injuries
dc.subjectMicroscopy, Fluorescence
dc.subjectRadiation Tolerance
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectMiddle Aged
dc.subjectFemale
dc.subjectTumor Suppressor Protein p53
dc.subjectDNA Breaks, Double-Stranded
dc.subjectRandomized Controlled Trials as Topic
dc.titleCorrelation between the radiation responses of fibroblasts cultured from individual patients and the risk of late reaction after breast radiotherapy.
dc.typeJournal Article
dcterms.dateAccepted2016-02-23
rioxxterms.versionofrecord10.1016/j.canlet.2016.02.036
rioxxterms.licenseref.urihttps://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-05
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCancer letters
pubs.issue2
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Translational Breast Radiobiology
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Translational Breast Radiobiology
pubs.publication-statusPublished
pubs.volume374
pubs.embargo.termsNot known
icr.researchteamTranslational Breast Radiobiologyen_US
dc.contributor.icrauthorYarnold, Johnen
dc.contributor.icrauthorGothard, Loneen
dc.contributor.icrauthorSomaiah, Navitaen


Files in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record