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dc.contributor.authorNuta, Oen_US
dc.contributor.authorSomaiah, Nen_US
dc.contributor.authorBoyle, Sen_US
dc.contributor.authorChua, MLen_US
dc.contributor.authorGothard, Len_US
dc.contributor.authorYarnold, Jen_US
dc.contributor.authorRothkamm, Ken_US
dc.contributor.authorHerskind, Cen_US
dc.date.accessioned2017-03-09T14:21:33Z
dc.date.issued2016-05en_US
dc.identifier.citationCancer letters, 2016, 374 (2), pp. 324 - 330en_US
dc.identifier.issn0304-3835en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/480
dc.identifier.eissn1872-7980en_US
dc.identifier.doi10.1016/j.canlet.2016.02.036en_US
dc.description.abstractLate normal tissue toxicity varies widely between patients and limits breast radiotherapy dose. Here we aimed to determine its relationship to DNA damage responses of fibroblast cultures from individual patients. Thirty-five breast cancer patients, with minimal or marked breast changes after breast-conserving therapy consented to receive a 4 Gy test irradiation to a small skin field of the left buttock and have punch biopsies taken from irradiated and unirradiated skin. Early-passage fibroblast cultures were established by outgrowth and irradiated in vitro with 0 or 4 Gy. 53BP1 foci, p53 and p21/CDKN1A were detected by immunofluorescence microscopy. Residual 53BP1 foci counts 24 h after in vitro irradiation were significantly higher in fibroblasts from RT-sensitive versus RT-resistant patients. Furthermore, significantly larger fractions of p53- but not p21/CDKN1A-positive fibroblasts were found in cultures from RT-sensitive patients without in vitro irradiation, and 2 h and 6 d post-irradiation. Exploratory analysis showed a stronger p53 response 2 h after irradiation of fibroblasts established from patients with severe reaction. These results associate the radiation response of fibroblasts with late reaction of the breast after RT and suggest a correlation with severity.en_US
dc.formatPrint-Electronicen_US
dc.format.extent324 - 330en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
dc.subjectCells, Cultureden_US
dc.subjectFibroblastsen_US
dc.subjectHumansen_US
dc.subjectBreast Neoplasmsen_US
dc.subjectRadiation Injuriesen_US
dc.subjectMicroscopy, Fluorescenceen_US
dc.subjectRadiation Toleranceen_US
dc.subjectAgeden_US
dc.subjectAged, 80 and overen_US
dc.subjectMiddle Ageden_US
dc.subjectFemaleen_US
dc.subjectTumor Suppressor Protein p53en_US
dc.subjectDNA Breaks, Double-Strandeden_US
dc.subjectRandomized Controlled Trials as Topicen_US
dc.titleCorrelation between the radiation responses of fibroblasts cultured from individual patients and the risk of late reaction after breast radiotherapy.en_US
dc.typeJournal Article
dcterms.dateAccepted2016-02-23en_US
rioxxterms.versionofrecord10.1016/j.canlet.2016.02.036en_US
rioxxterms.licenseref.startdate2016-05en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfCancer lettersen_US
pubs.issue2en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Targeted Therapy
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Clinical Academic Radiotherapy (Yarnold)
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Targeted Therapy
pubs.volume374en_US
pubs.embargo.termsNot knownen_US
icr.researchteamClinical Academic Radiotherapy (Yarnold)en_US
icr.researchteamTargeted Therapyen_US
dc.contributor.icrauthorYarnold, Johnen_US
dc.contributor.icrauthorGothard, Loneen_US
dc.contributor.icrauthorSomaiah, Navitaen_US


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