dc.contributor.author | Cagnet, S | |
dc.contributor.author | Ataca, D | |
dc.contributor.author | Sflomos, G | |
dc.contributor.author | Aouad, P | |
dc.contributor.author | Schuepbach-Mallepell, S | |
dc.contributor.author | Hugues, H | |
dc.contributor.author | Krust, A | |
dc.contributor.author | Ayyanan, A | |
dc.contributor.author | Scabia, V | |
dc.contributor.author | Brisken, C | |
dc.date.accessioned | 2021-11-19T09:17:08Z | |
dc.date.available | 2021-11-19T09:17:08Z | |
dc.date.issued | 2018-11-09 | |
dc.identifier.citation | Nature communications, 2018, 9 (1), pp. 4723 - ? | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/4885 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-018-07175-0 | |
dc.description.abstract | Oestrogen receptor α (ERα) is a transcription factor with ligand-independent and ligand-dependent activation functions (AF)-1 and -2. Oestrogens control postnatal mammary gland development acting on a subset of mammary epithelial cells (MECs), termed sensor cells, which are ERα-positive by immunohistochemistry (IHC) and secrete paracrine factors, which stimulate ERα-negative responder cells. Here we show that deletion of AF-1 or AF-2 blocks pubertal ductal growth and subsequent development because both are required for expression of essential paracrine mediators. Thirty percent of the luminal cells are ERα-negative by IHC but express Esr1 transcripts. This low level ERα expression through AF-2 is essential for cell expansion during puberty and growth-inhibitory during pregnancy. Cell-intrinsic ERα is not required for cell proliferation nor for secretory differentiation but controls transcript levels of cell motility and cell adhesion genes and a stem cell and epithelial mesenchymal transition (EMT) signature identifying ERα as a key regulator of mammary epithelial cell plasticity. | |
dc.format | Electronic | |
dc.format.extent | 4723 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Endocrine System | |
dc.subject | Epithelium | |
dc.subject | Mammary Glands, Animal | |
dc.subject | Epithelial Cells | |
dc.subject | Animals | |
dc.subject | Mice, Inbred C57BL | |
dc.subject | Steroids | |
dc.subject | Estrogen Receptor alpha | |
dc.subject | RNA, Messenger | |
dc.subject | Cell Proliferation | |
dc.subject | Gene Expression Regulation | |
dc.subject | Structure-Activity Relationship | |
dc.subject | Pregnancy | |
dc.subject | Phenotype | |
dc.subject | Female | |
dc.subject | Protein Domains | |
dc.title | Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2018-10-15 | |
rioxxterms.versionofrecord | 10.1038/s41467-018-07175-0 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2018-11-09 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Nature communications | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Endocrine control mechanisms | |
pubs.publication-status | Published | |
pubs.volume | 9 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Endocrine control mechanisms | |
dc.contributor.icrauthor | Brisken, Cathrin | |