Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells.
Date
2022-06-10Author
Berlak, M
Tucker, E
Dorel, M
Winkler, A
McGearey, A
Rodriguez-Fos, E
da Costa, BM
Barker, K
Fyle, E
Calton, E
Eising, S
Ober, K
Hughes, D
Koutroumanidou, E
Carter, P
Stankunaite, R
Proszek, P
Jain, N
Rosswog, C
Dorado-Garcia, H
Molenaar, JJ
Hubank, M
Barone, G
Anderson, J
Lang, P
Deubzer, HE
Künkele, A
Fischer, M
Eggert, A
Kloft, C
Henssen, AG
Boettcher, M
Hertwig, F
Blüthgen, N
Chesler, L
Schulte, JH
Type
Journal Article
Metadata
Show full item recordAbstract
BACKGROUND: Development of resistance to targeted therapies has tempered initial optimism that precision oncology would improve poor outcomes for cancer patients. Resistance mechanisms, however, can also confer new resistance-specific vulnerabilities, termed collateral sensitivities. Here we investigated anaplastic lymphoma kinase (ALK) inhibitor resistance in neuroblastoma, a childhood cancer frequently affected by activating ALK alterations. METHODS: Genome-wide forward genetic CRISPR-Cas9 based screens were performed to identify genes associated with ALK inhibitor resistance in neuroblastoma cell lines. Furthermore, the neuroblastoma cell line NBLW-R was rendered resistant by continuous exposure to ALK inhibitors. Genes identified to be associated with ALK inhibitor resistance were further investigated by generating suitable cell line models. In addition, tumor and liquid biopsy samples of four patients with ALK-mutated neuroblastomas before ALK inhibitor treatment and during tumor progression under treatment were genomically profiled. RESULTS: Both genome-wide CRISPR-Cas9-based screens and preclinical spontaneous ALKi resistance models identified NF1 loss and activating NRASQ61K mutations to confer resistance to chemically diverse ALKi. Moreover, human neuroblastomas recurrently developed de novo loss of NF1 and activating RAS mutations after ALKi treatment, leading to therapy resistance. Pathway-specific perturbations confirmed that NF1 loss and activating RAS mutations lead to RAS-MAPK signaling even in the presence of ALKi. Intriguingly, NF1 loss rendered neuroblastoma cells hypersensitive to MEK inhibition. CONCLUSIONS: Our results provide a clinically relevant mechanistic model of ALKi resistance in neuroblastoma and highlight new clinically actionable collateral sensitivities in resistant cells.
Collections
Subject
ALK
CRISPR screening
Ceritinib
Collateral sensitivity
Lorlatinib
NF1
NRAS
Neuroblastoma
Resistance
Trametinib
Anaplastic Lymphoma Kinase
Cell Line, Tumor
Child
Humans
Mutation
Neuroblastoma
Precision Medicine
Protein Kinase Inhibitors
Signal Transduction
Research team
Translational Genomics
Paediatric Tumour Biology
Language
eng
Date accepted
2022-04-22
License start date
2022-06-10
Citation
Molecular Cancer, 2022, 21 (1), pp. 126 -
Publisher
BMC