Show simple item record

dc.contributor.authorMessal, HA
dc.contributor.authorAlmagro, J
dc.contributor.authorZaw Thin, M
dc.contributor.authorTedeschi, A
dc.contributor.authorCiccarelli, A
dc.contributor.authorBlackie, L
dc.contributor.authorAnderson, KI
dc.contributor.authorMiguel-Aliaga, I
dc.contributor.authorvan Rheenen, J
dc.contributor.authorBehrens, A
dc.coverage.spatialEngland
dc.date.accessioned2022-09-16T10:15:45Z
dc.date.available2022-09-16T10:15:45Z
dc.date.issued2020-11-27
dc.identifier10.1038/s41596-020-00414-z
dc.identifier.citationNature Protocols, 2020, 16 (1), pp. 239 - 262en_US
dc.identifier.issn1754-2189
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/5496
dc.identifier.eissn1750-2799
dc.identifier.eissn1750-2799
dc.identifier.doi10.1038/s41596-020-00414-z
dc.identifier.doi10.1038/s41596-020-00414-z
dc.description.abstractAdvances in light-sheet and confocal microscopy now allow imaging of cleared large biological tissue samples and enable the 3D appreciation of cell and protein localization in their native organ environment. However, the sample preparations for such imaging are often onerous, and their capability for antigen detection is limited. Here, we describe FLASH (fast light-microscopic analysis of antibody-stained whole organs), a simple, rapid, fully customizable technique for molecular phenotyping of intact tissue volumes. FLASH utilizes non-degradative epitope recovery and membrane solubilization to enable the detection of a multitude of membranous, cytoplasmic and nuclear antigens in whole mouse organs and embryos, human biopsies, organoids and Drosophila. Retrieval and immunolabeling of epithelial markers, an obstacle for previous clearing techniques, can be achieved with FLASH. Upon volumetric imaging, FLASH-processed samples preserve their architecture and integrity and can be paraffin-embedded for subsequent histopathological analysis. The technique can be performed by scientists trained in light microscopy and yields results in <1 week.
dc.formatPrint-Electronic
dc.format.extent239 - 262
dc.languageeng
dc.language.isoengen_US
dc.publisherNATURE RESEARCHen_US
dc.relation.ispartofNature Protocols
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
dc.subjectAnimals
dc.subjectAntigens
dc.subjectDrosophila
dc.subjectEpitopes
dc.subjectFemale
dc.subjectFluorescent Antibody Technique
dc.subjectHumans
dc.subjectImaging, Three-Dimensional
dc.subjectKidney
dc.subjectLacrimal Apparatus
dc.subjectLiver
dc.subjectLung
dc.subjectMale
dc.subjectMammary Glands, Human
dc.subjectMice
dc.subjectMicroscopy, Confocal
dc.subjectOrganoids
dc.subjectPancreas
dc.subjectStomach
dc.titleAntigen retrieval and clearing for whole-organ immunofluorescence by FLASH.en_US
dc.typeJournal Article
dcterms.dateAccepted2020-09-18
dc.date.updated2022-09-16T10:14:53Z
rioxxterms.versionAMen_US
rioxxterms.versionofrecord10.1038/s41596-020-00414-zen_US
rioxxterms.licenseref.startdate2020-11-27
rioxxterms.typeJournal Article/Reviewen_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33247285
pubs.issue1
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Cancer Stem Cell
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1038/s41596-020-00414-z
pubs.volume16
icr.researchteamCancer Stem Cellen_US
icr.researchteamConvergence SC Managementen_US
dc.contributor.icrauthorZaw Thin, May
dc.contributor.icrauthorBehrens, Axel
icr.provenanceDeposited by Mr Arek Surman on 2022-09-16. Deposit type is initial. No. of files: 1. Files: Messaletal.pdf


Files in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record