dc.contributor.author | Xie, J | |
dc.contributor.author | Aiello, U | |
dc.contributor.author | Clement, Y | |
dc.contributor.author | Haidara, N | |
dc.contributor.author | Girbig, M | |
dc.contributor.author | Schmitzova, J | |
dc.contributor.author | Pena, V | |
dc.contributor.author | Müller, CW | |
dc.contributor.author | Libri, D | |
dc.contributor.author | Porrua, O | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2022-09-26T14:05:37Z | |
dc.date.available | 2022-09-26T14:05:37Z | |
dc.date.issued | 2022-07-15 | |
dc.identifier.citation | Science Advances, 2022, 8 (28), pp. eabm9875 - | en_US |
dc.identifier.issn | 2375-2548 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5501 | |
dc.identifier.eissn | 2375-2548 | |
dc.identifier.eissn | 2375-2548 | |
dc.identifier.doi | 10.1126/sciadv.abm9875 | |
dc.description.abstract | RNA polymerase III (RNAPIII) synthesizes essential and abundant noncoding RNAs such as transfer RNAs. Controlling RNAPIII span of activity by accurate and efficient termination is a challenging necessity to ensure robust gene expression and to prevent conflicts with other DNA-associated machineries. The mechanism of RNAPIII termination is believed to be simpler than that of other eukaryotic RNA polymerases, solely relying on the recognition of a T-tract in the nontemplate strand. Here, we combine high-resolution genome-wide analyses and in vitro transcription termination assays to revisit the mechanism of RNAPIII transcription termination in budding yeast. We show that T-tracts are necessary but not always sufficient for termination and that secondary structures of the nascent RNAs are important auxiliary cis-acting elements. Moreover, we show that the helicase Sen1 plays a key role in a fail-safe termination pathway. Our results provide a comprehensive model illustrating how multiple mechanisms cooperate to ensure efficient RNAPIII transcription termination. | |
dc.format | Print-Electronic | |
dc.format.extent | eabm9875 - | |
dc.language | eng | |
dc.language.iso | eng | en_US |
dc.publisher | American Association for the Advancement of Science (AAAS) | en_US |
dc.relation.ispartof | Science Advances | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.subject | DNA Helicases | |
dc.subject | Genome-Wide Association Study | |
dc.subject | RNA Polymerase III | |
dc.subject | Saccharomyces cerevisiae Proteins | |
dc.subject | Transcription, Genetic | |
dc.title | An integrated model for termination of RNA polymerase III transcription. | en_US |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-05-26 | |
dc.date.updated | 2022-09-26T13:01:46Z | |
rioxxterms.version | VoR | en_US |
rioxxterms.versionofrecord | 10.1126/sciadv.abm9875 | en_US |
rioxxterms.licenseref.startdate | 2022-07-15 | |
rioxxterms.type | Journal Article/Review | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/35857496 | |
pubs.issue | 28 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Structural Biology/Mechanisms and regulation of pre-mRNA splicing | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1126/sciadv.abm9875 | |
pubs.volume | 8 | |
icr.researchteam | Mech of pre-mRNA splicing | en_US |
dc.contributor.icrauthor | Pena, Vladimir | |
icr.provenance | Deposited by Prof Vlad Pena on 2022-09-26. Deposit type is initial. No. of files: 1. Files: An integrated model for termination of RNA polymerase III transcription.pdf | |