Show simple item record

dc.contributor.authorXie, J
dc.contributor.authorAiello, U
dc.contributor.authorClement, Y
dc.contributor.authorHaidara, N
dc.contributor.authorGirbig, M
dc.contributor.authorSchmitzova, J
dc.contributor.authorPena, V
dc.contributor.authorMüller, CW
dc.contributor.authorLibri, D
dc.contributor.authorPorrua, O
dc.coverage.spatialUnited States
dc.date.accessioned2022-09-26T14:05:37Z
dc.date.available2022-09-26T14:05:37Z
dc.date.issued2022-07-15
dc.identifier.citationScience Advances, 2022, 8 (28), pp. eabm9875 -en_US
dc.identifier.issn2375-2548
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/5501
dc.identifier.eissn2375-2548
dc.identifier.eissn2375-2548
dc.identifier.doi10.1126/sciadv.abm9875
dc.identifier.doi10.1126/sciadv.abm9875
dc.description.abstractRNA polymerase III (RNAPIII) synthesizes essential and abundant noncoding RNAs such as transfer RNAs. Controlling RNAPIII span of activity by accurate and efficient termination is a challenging necessity to ensure robust gene expression and to prevent conflicts with other DNA-associated machineries. The mechanism of RNAPIII termination is believed to be simpler than that of other eukaryotic RNA polymerases, solely relying on the recognition of a T-tract in the nontemplate strand. Here, we combine high-resolution genome-wide analyses and in vitro transcription termination assays to revisit the mechanism of RNAPIII transcription termination in budding yeast. We show that T-tracts are necessary but not always sufficient for termination and that secondary structures of the nascent RNAs are important auxiliary cis-acting elements. Moreover, we show that the helicase Sen1 plays a key role in a fail-safe termination pathway. Our results provide a comprehensive model illustrating how multiple mechanisms cooperate to ensure efficient RNAPIII transcription termination.
dc.formatPrint-Electronic
dc.format.extenteabm9875 -
dc.languageeng
dc.language.isoengen_US
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.ispartofScience Advances
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectDNA Helicases
dc.subjectGenome-Wide Association Study
dc.subjectRNA Polymerase III
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTranscription, Genetic
dc.titleAn integrated model for termination of RNA polymerase III transcription.en_US
dc.typeJournal Article
dcterms.dateAccepted2022-05-26
dc.date.updated2022-09-26T13:01:46Z
rioxxterms.versionVoRen_US
rioxxterms.versionofrecord10.1126/sciadv.abm9875en_US
rioxxterms.licenseref.startdate2022-07-15
rioxxterms.typeJournal Article/Reviewen_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35857496
pubs.issue28
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Structural Biology/Mechanisms and regulation of pre-mRNA splicing
pubs.publication-statusPublished
pubs.publisher-urlhttp://dx.doi.org/10.1126/sciadv.abm9875
pubs.volume8
icr.researchteamMech of pre-mRNA splicingen_US
dc.contributor.icrauthorPena, Vladimir
icr.provenanceDeposited by Prof Vlad Pena on 2022-09-26. Deposit type is initial. No. of files: 1. Files: An integrated model for termination of RNA polymerase III transcription.pdf


Files in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record

http://creativecommons.org/licenses/by/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/