An integrated model for termination of RNA polymerase III transcription.
Date
2022-07-15ICR Author
Author
Xie, J
Aiello, U
Clement, Y
Haidara, N
Girbig, M
Schmitzova, J
Pena, V
Müller, CW
Libri, D
Porrua, O
Type
Journal Article
Metadata
Show full item recordAbstract
RNA polymerase III (RNAPIII) synthesizes essential and abundant noncoding RNAs such as transfer RNAs. Controlling RNAPIII span of activity by accurate and efficient termination is a challenging necessity to ensure robust gene expression and to prevent conflicts with other DNA-associated machineries. The mechanism of RNAPIII termination is believed to be simpler than that of other eukaryotic RNA polymerases, solely relying on the recognition of a T-tract in the nontemplate strand. Here, we combine high-resolution genome-wide analyses and in vitro transcription termination assays to revisit the mechanism of RNAPIII transcription termination in budding yeast. We show that T-tracts are necessary but not always sufficient for termination and that secondary structures of the nascent RNAs are important auxiliary cis-acting elements. Moreover, we show that the helicase Sen1 plays a key role in a fail-safe termination pathway. Our results provide a comprehensive model illustrating how multiple mechanisms cooperate to ensure efficient RNAPIII transcription termination.
Collections
Subject
DNA Helicases
Genome-Wide Association Study
RNA Polymerase III
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Research team
Mech of pre-mRNA splicing
Language
eng
Date accepted
2022-05-26
License start date
2022-07-15
Citation
Science Advances, 2022, 8 (28), pp. eabm9875 -
Publisher
American Association for the Advancement of Science (AAAS)