dc.contributor.author | Humphries, MP | |
dc.contributor.author | Bingham, V | |
dc.contributor.author | Abdullah Sidi, F | |
dc.contributor.author | Craig, S | |
dc.contributor.author | Lara, B | |
dc.contributor.author | El-Daly, H | |
dc.contributor.author | O'Doherty, N | |
dc.contributor.author | Maxwell, P | |
dc.contributor.author | Lewis, C | |
dc.contributor.author | McQuaid, S | |
dc.contributor.author | Lyness, J | |
dc.contributor.author | James, J | |
dc.contributor.author | Snead, DRJ | |
dc.contributor.author | Salto-Tellez, M | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2023-04-28T11:44:34Z | |
dc.date.available | 2023-04-28T11:44:34Z | |
dc.date.issued | 2023-01-30 | |
dc.identifier | jcp-2022-208525 | |
dc.identifier.citation | Journal of Clinical Pathology, 2023, pp. jcp-2022-208525 - | |
dc.identifier.issn | 0021-9746 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5764 | |
dc.identifier.eissn | 1472-4146 | |
dc.identifier.eissn | 1472-4146 | |
dc.identifier.doi | 10.1136/jcp-2022-208525 | |
dc.description.abstract | Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF. | |
dc.format | Print-Electronic | |
dc.format.extent | jcp-2022-208525 - | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | BMJ PUBLISHING GROUP | |
dc.relation.ispartof | Journal of Clinical Pathology | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Autopsy | |
dc.subject | COVID-19 | |
dc.subject | MOLECULAR BIOLOGY | |
dc.subject | Pathology, Molecular | |
dc.title | Technical note on the exploration of COVID-19 in autopsy material. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-10-17 | |
dc.date.updated | 2023-04-28T11:43:52Z | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | 10.1136/jcp-2022-208525 | |
rioxxterms.licenseref.startdate | 2023-01-30 | |
rioxxterms.type | Journal Article/Review | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/36717223 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Integrated Pathology | |
pubs.organisational-group | /ICR/ImmNet | |
pubs.publication-status | Published online | |
pubs.publisher-url | http://dx.doi.org/10.1136/jcp-2022-208525 | |
icr.researchteam | Integrated Pathology | |
dc.contributor.icrauthor | Salto-Tellez, Manuel | |
icr.provenance | Deposited by Mr Arek Surman (impersonating Dr Ragu Ratnakumaran) on 2023-04-28. Deposit type is initial. No. of files: 1. Files: jcp-2022-208525.full.pdf | |