Molecular basis for the bifunctional Uba4-Urm1 sulfur-relay system in tRNA thiolation and ubiquitin-like conjugation.
Date
2020-10-01Author
Pabis, M
Termathe, M
Ravichandran, KE
Kienast, SD
Krutyhołowa, R
Sokołowski, M
Jankowska, U
Grudnik, P
Leidel, SA
Glatt, S
Type
Journal Article
Metadata
Show full item recordAbstract
The chemical modification of tRNA bases by sulfur is crucial to tune translation and to optimize protein synthesis. In eukaryotes, the ubiquitin-related modifier 1 (Urm1) pathway is responsible for the synthesis of 2-thiolated wobble uridine (U34 ). During the key step of the modification cascade, the E1-like activating enzyme ubiquitin-like protein activator 4 (Uba4) first adenylates and thiocarboxylates the C-terminus of its substrate Urm1. Subsequently, activated thiocarboxylated Urm1 (Urm1-COSH) can serve as a sulfur donor for specific tRNA thiolases or participate in ubiquitin-like conjugation reactions. Structural and mechanistic details of Uba4 and Urm1 have remained elusive but are key to understand the evolutionary branch point between ubiquitin-like proteins (UBL) and sulfur-relay systems. Here, we report the crystal structures of full-length Uba4 and its heterodimeric complex with its substrate Urm1. We show how the two domains of Uba4 orchestrate recognition, binding, and thiocarboxylation of the C-terminus of Urm1. Finally, we uncover how the catalytic domains of Uba4 communicate efficiently during the reaction cycle and identify a mechanism that enables Uba4 to protect itself against self-conjugation with its own product, namely activated Urm1-COSH.
Collections
Subject
adenylation
tRNA modification
thioester
thiolation
ubiquitin-like proteins
Humans
Nucleotidyltransferases
RNA, Transfer
Sulfur
Sulfurtransferases
Ubiquitins
Language
eng
Date accepted
2020-06-26
License start date
2020-10-01
Citation
The EMBO Journal, 2020, 39 (19), pp. e105087 -
Publisher
WILEY