Expression of Mitochondrial Cytochrome C Oxidase Chaperone Gene (COX20) Improves Tolerance to Weak Acid and Oxidative Stress during Yeast Fermentation.
Date
2015-10-01Author
Kumar, V
Hart, AJ
Keerthiraju, ER
Waldron, PR
Tucker, GA
Greetham, D
Yang S
Type
Journal Article
Metadata
Show full item recordAbstract
INTRODUCTION: Saccharomyces cerevisiae is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pre-treatment of lignocellulosic material releases acetic acid and previous work identified a cytochrome oxidase chaperone gene (COX20) which was significantly up-regulated in yeast cells in the presence of acetic acid. RESULTS: A Δcox20 strain was sensitive to the presence of acetic acid compared with the background strain. Overexpressing COX20 using a tetracycline-regulatable expression vector system in a Δcox20 strain, resulted in tolerance to the presence of acetic acid and tolerance could be ablated with addition of tetracycline. Assays also revealed that overexpression improved tolerance to the presence of hydrogen peroxide-induced oxidative stress. CONCLUSION: This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.
Collections
Subject
Acetic Acid
Fermentation
Gene Expression Regulation, Fungal
Hydrogen Peroxide
Hydrolysis
Membrane Proteins
Mitochondrial Proteins
Oxidative Stress
Phenotype
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Tetracycline
Triticum
Language
eng
Date accepted
2015-09-08
License start date
2015-10-01
Citation
PLoS One, 2015, 10 (10), pp. e0139129 -
Publisher
PUBLIC LIBRARY SCIENCE