dc.contributor.author | Davis, RW | |
dc.contributor.author | Snyder, E | |
dc.contributor.author | Miller, J | |
dc.contributor.author | Carter, S | |
dc.contributor.author | Houser, C | |
dc.contributor.author | Klampatsa, A | |
dc.contributor.author | Albelda, SM | |
dc.contributor.author | Cengel, KA | |
dc.contributor.author | Busch, TM | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2024-05-20T10:45:04Z | |
dc.date.available | 2024-05-20T10:45:04Z | |
dc.date.issued | 2019-01-01 | |
dc.identifier.citation | Photochemistry and Photobiology, 2019, 95 (1), pp. 430 - 438 | |
dc.identifier.issn | 0031-8655 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/6228 | |
dc.identifier.eissn | 1751-1097 | |
dc.identifier.eissn | 1751-1097 | |
dc.identifier.doi | 10.1111/php.13040 | |
dc.identifier.doi | 10.1111/php.13040 | |
dc.description.abstract | Inflammatory cells, most especially neutrophils, can be a necessary component of the antitumor activity occurring after administration of photodynamic therapy. Generation of neutrophil responses has been suggested to be particularly important in instances when the delivered photodynamic therapy (PDT) dose is insufficient. In these cases, the release of neutrophil granules and engagement of antitumor immunity may play an important role in eliminating residual disease. Herein, we utilize in vivo imaging of luminol chemiluminescence to noninvasively monitor neutrophil activation after PDT administration. Studies were performed in the AB12 murine model of mesothelioma, treated with Photofrin-PDT. Luminol-generated chemiluminescence increased transiently 1 h after PDT, followed by a subsequent decrease at 4 h after PDT. The production of luminol signal was not associated with the influx of Ly6G+ cells, but was related to oxidative burst, as an indicator of neutrophil function. Most importantly, greater levels of luminol chemiluminescence 1 h after PDT were prognostic of a complete response at 90 days after PDT. Taken together, this research supports an important role for early activity by Ly6G+ cells in the generation of long-term PDT responses in mesothelioma, and it points to luminol chemiluminescence as a potentially useful approach for preclinical monitoring of neutrophil activation by PDT. | |
dc.format | Print-Electronic | |
dc.format.extent | 430 - 438 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | WILEY | |
dc.relation.ispartof | Photochemistry and Photobiology | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Animals | |
dc.subject | Biomarkers | |
dc.subject | Dihematoporphyrin Ether | |
dc.subject | Luminescence | |
dc.subject | Luminol | |
dc.subject | Mesothelioma | |
dc.subject | Mice | |
dc.subject | Mice, Inbred BALB C | |
dc.subject | Neutrophil Activation | |
dc.subject | Neutrophils | |
dc.subject | Photochemotherapy | |
dc.subject | Photosensitizing Agents | |
dc.subject | Prognosis | |
dc.title | Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2018-10-03 | |
dc.date.updated | 2024-05-14T12:30:18Z | |
rioxxterms.version | AM | |
rioxxterms.versionofrecord | 10.1111/php.13040 | |
rioxxterms.licenseref.startdate | 2019-01-01 | |
rioxxterms.type | Journal Article/Review | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/30357853 | |
pubs.issue | 1 | |
pubs.organisational-group | ICR | |
pubs.organisational-group | ICR/Primary Group | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Therapeutics | |
pubs.organisational-group | ICR/Primary Group/ICR Divisions/Cancer Therapeutics/Thoracic Oncology Immunotherapy Group (TOIG) | |
pubs.organisational-group | ICR/ImmNet | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1111/php.13040 | |
pubs.volume | 95 | |
icr.researchteam | Thor Onco Immuno Group | |
dc.contributor.icrauthor | Klampatsa, Astero | |
icr.provenance | Deposited by Dr Astero Klampatsa on 2024-05-14. Deposit type is initial. No. of files: 1. Files: Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Th.pdf | |