Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy.
Date
2019-01-01ICR Author
Author
Davis, RW
Snyder, E
Miller, J
Carter, S
Houser, C
Klampatsa, A
Albelda, SM
Cengel, KA
Busch, TM
Type
Journal Article
Metadata
Show full item recordAbstract
Inflammatory cells, most especially neutrophils, can be a necessary component of the antitumor activity occurring after administration of photodynamic therapy. Generation of neutrophil responses has been suggested to be particularly important in instances when the delivered photodynamic therapy (PDT) dose is insufficient. In these cases, the release of neutrophil granules and engagement of antitumor immunity may play an important role in eliminating residual disease. Herein, we utilize in vivo imaging of luminol chemiluminescence to noninvasively monitor neutrophil activation after PDT administration. Studies were performed in the AB12 murine model of mesothelioma, treated with Photofrin-PDT. Luminol-generated chemiluminescence increased transiently 1 h after PDT, followed by a subsequent decrease at 4 h after PDT. The production of luminol signal was not associated with the influx of Ly6G+ cells, but was related to oxidative burst, as an indicator of neutrophil function. Most importantly, greater levels of luminol chemiluminescence 1 h after PDT were prognostic of a complete response at 90 days after PDT. Taken together, this research supports an important role for early activity by Ly6G+ cells in the generation of long-term PDT responses in mesothelioma, and it points to luminol chemiluminescence as a potentially useful approach for preclinical monitoring of neutrophil activation by PDT.
Collections
Subject
Animals
Biomarkers
Dihematoporphyrin Ether
Luminescence
Luminol
Mesothelioma
Mice
Mice, Inbred BALB C
Neutrophil Activation
Neutrophils
Photochemotherapy
Photosensitizing Agents
Prognosis
Research team
Thor Onco Immuno Group
Language
eng
Date accepted
2018-10-03
License start date
2019-01-01
Citation
Photochemistry and Photobiology, 2019, 95 (1), pp. 430 - 438
Publisher
WILEY