Browsing Molecular Pathology by author "Krasny, Lukas"
Now showing items 1-9 of 9
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3D Functional Genomics Screens Identify CREBBP as a Targetable Driver in Aggressive Triple-Negative Breast Cancer.
Peck, B; Bland, P; Mavrommati, I; Muirhead, G; Cottom, H; et al. (AMER ASSOC CANCER RESEARCH, 2021-02-15)Triple-negative breast cancers (TNBC) are resistant to standard-of-care chemotherapy and lack known targetable driver gene alterations. Identification of novel drivers could aid the discovery of new treatment strategies ... -
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.
Krasny, L; Bland, P; Burns, J; Lima, NC; Harrison, PT; et al. (COMPANY BIOLOGISTS LTD, 2020-06-03)SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that ... -
Advances in the proteomic profiling of the matrisome and adhesome.
Krasny, L; Huang, PH (TAYLOR & FRANCIS LTD, 2021-09-02)INTRODUCTION: The matrisome and adhesome comprise proteins that are found within or are associated with the extracellular matrix (ECM) and adhesion complexes, respectively. Interactions between cells and their microenvironment ... -
Comparative proteomic assessment of matrisome enrichment methodologies.
Krasny, L; Paul, A; Wai, P; Howard, BA; Natrajan, RC; et al. (PORTLAND PRESS LTD, 2016-11-01)The matrisome is a complex and heterogeneous collection of extracellular matrix (ECM) and ECM-associated proteins that play important roles in tissue development and homeostasis. While several strategies for matrisome ... -
Data-independent acquisition mass spectrometry (DIA-MS) for proteomic applications in oncology.
Krasny, L; Huang, PH (ROYAL SOC CHEMISTRY, 2020-10-09)Data-independent acquisition mass spectrometry (DIA-MS) is a next generation proteomic methodology that generates permanent digital proteome maps offering highly reproducible retrospective analysis of cellular and tissue ... -
Fibroblastic Reticular Cells Control Conduit Matrix Deposition during Lymph Node Expansion.
Martinez, VG; Pankova, V; Krasny, L; Singh, T; Makris, S; et al. (CELL PRESS, 2019-11-26)Lymph nodes (LNs) act as filters, constantly sampling peripheral cues. This is facilitated by the conduit network, a tubular structure of aligned extracellular matrix (ECM) fibrils ensheathed by fibroblastic reticular cells ... -
Proteomic profiling of soft tissue sarcomas with SWATH mass spectrometry.
Milighetti, M; Krasny, L; Lee, ATJ; Morani, G; Szecsei, C; et al. (ELSEVIER, 2021-06-15)Soft tissue sarcomas (STS) are a group of rare and heterogeneous cancers. While large-scale genomic and epigenomic profiling of STS have been undertaken, proteomic analysis has thus far been limited. Here we utilise ... -
Spatial localisation of Discoidin Domain Receptor 2 (DDR2) signalling is dependent on its collagen binding and kinase activity.
Luczynski, MT; Harrison, PT; Lima, N; Krasny, L; Paul, A; et al. (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018-06-18)Discoidin Domain Receptor 2 (DDR2) is a collagen-binding receptor tyrosine kinase that initiates delayed and sustained tyrosine phosphorylation signalling. To understand the molecular basis of this unique phosphorylation ... -
SWATH mass spectrometry as a tool for quantitative profiling of the matrisome.
Krasny, L; Bland, P; Kogata, N; Wai, P; Howard, BA; et al. (ELSEVIER, 2018-03-01)Proteomic analysis of extracellular matrix (ECM) and ECM-associated proteins, collectively known as the matrisome, is a challenging task due to the inherent complexity and insolubility of these proteins. Here we present ...