dc.contributor.author | Shah, A | |
dc.contributor.author | Delgado-Goni, T | |
dc.contributor.author | Casals Galobart, T | |
dc.contributor.author | Wantuch, S | |
dc.contributor.author | Jamin, Y | |
dc.contributor.author | Leach, MO | |
dc.contributor.author | Robinson, SP | |
dc.contributor.author | Bamber, J | |
dc.contributor.author | Beloueche-Babari, M | |
dc.date.accessioned | 2017-07-19T11:55:33Z | |
dc.date.issued | 2017-08-15 | |
dc.identifier.citation | Scientific reports, 2017, 7 (1), pp. 8215 - ? | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/711 | |
dc.identifier.eissn | 2045-2322 | |
dc.identifier.doi | 10.1038/s41598-017-07864-8 | |
dc.description.abstract | Targeted therapies specific to the BRAF-MEK-ERK signaling pathway have shown great promise in the treatment of malignant melanoma in the last few years, with these drugs now commonly used in clinic. Melanoma cells treated using these agents are known to exhibit increased levels of melanin pigment and tyrosinase activity. In this study we assessed the potential of non-invasive imaging approaches (photoacoustic imaging (PAI) and magnetic resonance imaging (MRI)) to detect melanin induction in SKMEL28 human melanoma cells, following inhibition of Hsp90 and BRAF signaling using 17-AAG and vemurafenib, respectively. We confirmed, using western blot and spectrophotometry, that Hsp90 or BRAF inhibitor-induced melanoma cell differentiation resulted in an upregulation of tyrosinase and melanin expression levels, in comparison to control cells. This post-treatment increase in cellular pigmentation induced a significant increase in PAI signals that are spectrally identifiable and shortening of the MRI relaxation times T 1 and [Formula: see text]. This proof-of-concept study demonstrates the potential of MRI and PAI for detecting the downstream cellular changes induced by Hsp90 and BRAF-MEK-targeted therapies in melanoma cells with potential significance for in vivo imaging. | |
dc.format | Electronic | |
dc.format.extent | 8215 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cell Line, Tumor | |
dc.subject | Humans | |
dc.subject | Melanoma | |
dc.subject | Proto-Oncogene Proteins B-raf | |
dc.subject | Antineoplastic Agents | |
dc.subject | Protein Kinase Inhibitors | |
dc.subject | Magnetic Resonance Imaging | |
dc.subject | Cell Differentiation | |
dc.subject | Cell Proliferation | |
dc.subject | Pigments, Biological | |
dc.subject | HSP90 Heat-Shock Proteins | |
dc.subject | Photoacoustic Techniques | |
dc.title | Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-07-04 | |
rioxxterms.versionofrecord | 10.1038/s41598-017-07864-8 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2017-08-15 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Scientific reports | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Pre-Clinical MRI | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Ultrasound & Optical Imaging | |
pubs.organisational-group | /ICR/Primary Group/Royal Marsden Clinical Units | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Magnetic Resonance | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Pre-Clinical MRI | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Radiotherapy and Imaging/Ultrasound & Optical Imaging | |
pubs.organisational-group | /ICR/Primary Group/Royal Marsden Clinical Units | |
pubs.publication-status | Published | |
pubs.volume | 7 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Magnetic Resonance | |
icr.researchteam | Pre-Clinical MRI | |
icr.researchteam | Ultrasound & Optical Imaging | |
dc.contributor.icrauthor | Shah, Anant | |
dc.contributor.icrauthor | Casals Galobart, Maria | |
dc.contributor.icrauthor | Jamin, Yann | |
dc.contributor.icrauthor | Leach, Martin | |
dc.contributor.icrauthor | Robinson, Simon | |
dc.contributor.icrauthor | Bamber, Jeffrey | |
dc.contributor.icrauthor | Beloueche-Babari, Mounia | |