dc.contributor.author | Jungwirth, U | |
dc.contributor.author | van Weverwijk, A | |
dc.contributor.author | Melake, MJ | |
dc.contributor.author | Chambers, AF | |
dc.contributor.author | Gao, Q | |
dc.contributor.author | Fivaz, M | |
dc.contributor.author | Isacke, CM | |
dc.date.accessioned | 2017-11-22T16:23:02Z | |
dc.date.issued | 2018-01-18 | |
dc.identifier.citation | Disease models & mechanisms, 2018, 11 (1) | |
dc.identifier.issn | 1754-8403 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/935 | |
dc.identifier.eissn | 1754-8411 | |
dc.identifier.doi | 10.1242/dmm.031740 | |
dc.description.abstract | Studying the complex mechanisms underlying breast cancer metastasis and therapy response necessitates relevant in vivo models, particularly syngeneic models with an intact immune system. Two syngeneic spontaneously metastatic sublines, D2A1-m1 and D2A1-m2, were generated from the poorly metastasising BALB/c-derived D2A1 cell line by serial in vivo passaging. In vivo and in vitro analyses revealed distinct and shared characteristics of the metastatic D2A1-m1 and D2A1-m2 sublines. In particular, D2A1-m1 cells are more aggressive in experimental metastasis assays, while D2A1-m2 cells are more efficient at disseminating from the primary tumour in spontaneous metastasis assays. Surprisingly, classical metastasis-associated in vitro phenotypes, such as enhanced proliferation, migration and invasion, are reduced in the sublines compared to the parental cell line. Further, evasion of immune control cannot fully explain their enhanced metastatic properties. By contrast, both sublines show increased resistance to apoptosis when cultured in non-adherent conditions and, for the D2A1-m2 subline, increased 3D tumour spheroid growth. Moreover, the enhanced spontaneous metastatic phenotype of the D2A1-m2 subline is associated with an increased ability to recruit an activated tumour stroma. The metastatic D2A1-m1 and D2A1-m2 cell lines provide additional syngeneic models for investigating the different steps of the metastatic cascade and thereby represent valuable tools for breast cancer researchers. Finally, this study highlights that morphology and cell behaviour in 2D cell-based assays cannot be used as a reliable predictor of metastatic behaviour in vivo. | |
dc.format | Electronic | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | COMPANY BIOLOGISTS LTD | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cell Line, Tumor | |
dc.subject | Stromal Cells | |
dc.subject | Animals | |
dc.subject | Mice, Inbred BALB C | |
dc.subject | Mammary Neoplasms, Animal | |
dc.subject | Neoplasm Metastasis | |
dc.subject | Disease Models, Animal | |
dc.subject | Gene Expression Profiling | |
dc.subject | Cell Adhesion | |
dc.subject | Immunocompromised Host | |
dc.subject | Gene Expression Regulation, Neoplastic | |
dc.subject | Databases, Genetic | |
dc.subject | Female | |
dc.title | Generation and characterisation of two D2A1 mammary cancer sublines to model spontaneous and experimental metastasis in a syngeneic BALB/c host. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-11-17 | |
rioxxterms.funder | The Institute of Cancer Research | |
rioxxterms.identifier.project | Unspecified | |
rioxxterms.versionofrecord | 10.1242/dmm.031740 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2018-01-18 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Disease models & mechanisms | |
pubs.issue | 1 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Molecular Cell Biology | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Molecular Cell Biology | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
pubs.embargo.terms | Not known | |
icr.researchteam | Molecular Cell Biology | |
dc.contributor.icrauthor | Melake, Johanna Miriam | |
dc.contributor.icrauthor | Gao, Qiong | |
dc.contributor.icrauthor | Isacke, Clare | |