Browsing by author "Bakal, Christopher"
Now showing items 1-20 of 34
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A comprehensive model for the proliferation-quiescence decision in response to endogenous DNA damage in human cells.
Heldt, FS; Barr, AR; Cooper, S; Bakal, C; Novák, B (NATL ACAD SCIENCES, 2018-03-06)Human cells that suffer mild DNA damage can enter a reversible state of growth arrest known as quiescence. This decision to temporarily exit the cell cycle is essential to prevent the propagation of mutations, and most ... -
A Dynamical Framework for the All-or-None G1/S Transition.
Barr, AR; Heldt, FS; Zhang, T; Bakal, C; Novák, B (CELL PRESS, 2016-01-27)The transition from G1 into DNA replication (S phase) is an emergent behavior resulting from dynamic and complex interactions between cyclin-dependent kinases (Cdks), Cdk inhibitors (CKIs), and the anaphase-promoting ... -
A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
Stojic, L; Lun, ATL; Mascalchi, P; Ernst, C; Redmond, AM; et al. (NATURE PUBLISHING GROUP, 2020-04-15)Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more ... -
Accelerating Live Single-Cell Signalling Studies.
Cooper, S; Bakal, C (ELSEVIER SCIENCE LONDON, 2017-05-01)The dynamics of signalling networks that couple environmental conditions with cellular behaviour can now be characterised in exquisite detail using live single-cell imaging experiments. Recent improvements in our abilities ... -
Actin nucleators safeguard replication forks by limiting nascent strand degradation.
Nieminuszczy, J; Martin, PR; Broderick, R; Krwawicz, J; Kanellou, A; et al. (OXFORD UNIV PRESS, 2023-07-07)Accurate genome replication is essential for all life and a key mechanism of disease prevention, underpinned by the ability of cells to respond to replicative stress (RS) and protect replication forks. These responses rely ... -
AKT-mTOR signaling modulates the dynamics of IRE1 RNAse activity by regulating ER-mitochondria contacts.
Sanchez-Alvarez, M; Del Pozo, MA; Bakal, C (NATURE PORTFOLIO, 2017-11-28)Inositol Requiring Enzyme-1 (IRE1) is the most conserved transducer of the Unfolded Protein Response (UPR), a surveillance mechanism that ensures homeostasis of the endoplasmic reticulum (ER) in eukaryotes. IRE1 activation ... -
ALPK1 hotspot mutation as a driver of human spiradenoma and spiradenocarcinoma.
Rashid, M; van der Horst, M; Mentzel, T; Butera, F; Ferreira, I; et al. (NATURE PUBLISHING GROUP, 2019-05-17)Spiradenoma and cylindroma are distinctive skin adnexal tumors with sweat gland differentiation and potential for malignant transformation and aggressive behaviour. We present the genomic analysis of 75 samples from 57 ... -
ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion.
Bousgouni, V; Inge, O; Robertson, D; Jones, I; Clatworthy, I; et al. (CELL PRESS, 2022-08-19)Rho GTP Exchange Factors (RhoGEFs) and Rho GTPase Activating Proteins (RhoGAPs) are large families of molecules that regulate shape determination in all eukaryotes. In pathologies such as melanoma, RhoGEF and RhoGAP activity ... -
Arresting metastasis within the microcirculation.
Varotsos Vrynas, A; Perea Paizal, J; Bakal, C; Au, SH (SPRINGER, 2021-07-09)The behaviour of circulating tumour cells in the microcirculation remains poorly understood. Growing evidence suggests that biomechanical adaptations and interactions with blood components, i.e. immune cells and platelets ... -
Cell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells.
Sero, JE; Sailem, HZ; Ardy, RC; Almuttaqi, H; Zhang, T; et al. (WILEY, 2015-03-03)Although a great deal is known about the signaling events that promote nuclear translocation of NF-κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In ... -
Characterization of proteome-size scaling by integrative omics reveals mechanisms of proliferation control in cancer.
Jones, I; Dent, L; Higo, T; Roumeliotis, T; Arias Garcia, M; et al. (AMER ASSOC ADVANCEMENT SCIENCE, 2023-01-25)Almost all living cells maintain size uniformity through successive divisions. Proteins that over and underscale with size can act as rheostats, which regulate cell cycle progression. Using a multiomic strategy, we leveraged ... -
Clustering of Tir during enteropathogenic E. coli infection triggers calcium influx-dependent pyroptosis in intestinal epithelial cells.
Zhong, Q; Roumeliotis, TI; Kozik, Z; Cepeda-Molero, M; Fernández, LÁ; et al. (PUBLIC LIBRARY SCIENCE, 2020-12-30)Clustering of the enteropathogenic Escherichia coli (EPEC) type III secretion system (T3SS) effector translocated intimin receptor (Tir) by intimin leads to actin polymerisation and pyroptotic cell death in macrophages. ... -
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.
Hégarat, N; Crncec, A; Suarez Peredo Rodriguez, MF; Echegaray Iturra, F; Gu, Y; et al. (WILEY, 2020-06-02)Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute ... -
Discovery of Rare Phenotypes in Cellular Images Using Weakly Supervised Deep Learning
Sailem, H; Arias-Garcia, M; Bakal, C; Zisserman, A; Rittscher, J (IEEE, 2017-01-01) -
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.
Barr, AR; Cooper, S; Heldt, FS; Butera, F; Stoy, H; et al. (NATURE PUBLISHING GROUP, 2017-03-20)Following DNA damage caused by exogenous sources, such as ionizing radiation, the tumour suppressor p53 mediates cell cycle arrest via expression of the CDK inhibitor, p21. However, the role of p21 in maintaining genomic ... -
Dual-view oblique plane microscopy (dOPM).
Sparks, H; Dent, L; Bakal, C; Behrens, A; Salbreux, G; et al. (OPTICAL SOC AMER, 2020-12-01)We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a ... -
Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging.
Masuyama, K; Higo, T; Lee, J-K; Matsuura, R; Jones, I; et al. (NATURE PORTFOLIO, 2021-07-19)In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) ... -
Identification of clinically predictive metagenes that encode components of a network coupling cell shape to transcription by image-omics.
Sailem, HZ; Bakal, C (COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2017-02-01)The associations between clinical phenotypes (tumor grade, survival) and cell phenotypes, such as shape, signaling activity, and gene expression, are the basis for cancer pathology, but the mechanisms explaining these ... -
Identification of phenotype-specific networks from paired gene expression-cell shape imaging data.
Barker, CG; Petsalaki, E; Giudice, G; Sero, J; Ekpenyong, EN; et al. (COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2022-04-01)The morphology of breast cancer cells is often used as an indicator of tumor severity and prognosis. Additionally, morphology can be used to identify more fine-grained, molecular developments within a cancer cell, such as ... -
Mechanical and Systems Biology of Cancer.
Spill, F; Bakal, C; Mak, M (ELSEVIER SCIENCE BV, 2018-01-01)Mechanics and biochemical signaling are both often deregulated in cancer, leading toincreased cell invasiveness, proliferation, and survival. The dynamics and interactions of cytoskeletal components control basic mechanical ...