Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging.
Date
2021-07-19Author
Masuyama, K
Higo, T
Lee, J-K
Matsuura, R
Jones, I
Bakal, C
Higo, S
Morimoto, S
Miyagawa, S
Sawa, Y
Sakata, Y
Type
Journal Article
Metadata
Show full item recordAbstract
In contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Manipulation of cultured cardiomyocyte toward homogeneously aligned increased their contractility, suggesting that homogeneous alignment in DCM mice is due to a sort of alignment remodelling as a way to compensate cardiac dysfunction. Our findings provide the first intravital evidence of cardiomyocyte alignment and will bring new insights into understanding the mechanism of heart failure.
Collections
Subject
Animals
Animals, Newborn
Cardiomyopathy, Dilated
Cardiomyopathy, Hypertrophic
Cell Movement
Cells, Cultured
Diagnostic Imaging
Male
Mice
Mice, Transgenic
Myocytes, Cardiac
Rats
Rats, Wistar
Research team
Dynamical Cell Systems
Language
eng
Date accepted
2021-07-01
License start date
2021-07-19
Citation
Scientific Reports, 2021, 11 (1), pp. 14698 -
Publisher
NATURE PORTFOLIO