• Requirement for PBAF in transcriptional repression and repair at DNA breaks in actively transcribed regions of chromatin. 

  Kakarougkas, A; Ismail, A; Chambers, AL; Riballo, E; Herbert, AD; Künzel, J; Löbrich, M; Jeggo, PA; Downs, JA

  Actively transcribed regions of the genome are vulnerable to genomic instability. Recently, it was discovered that transcription is repressed in response to neighboring DNA double-strand breaks (DSBs). It is not known ...
• Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase. 

  Coster, G; Frigola, J; Beuron, F; Morris, EP; Diffley, JFX

  Loading of the six related Minichromosome Maintenance (MCM) proteins as head-to-head double hexamers during DNA replication origin licensing is crucial for ensuring once-per-cell-cycle DNA replication in eukaryotic cells. ...
• Sex specific retinoic acid signaling is required for the initiation of urogenital sinus bud development. 

  Bryant, SL; Francis, JC; Lokody, IB; Wang, H; Risbridger, GP; Loveland, KL; Swain, A (2014-11)

  The mammalian urogenital sinus (UGS) develops in a sex specific manner, giving rise to the prostate in the male and the sinus vagina in the embryonic female. Androgens, produced by the embryonic testis, have been shown to ...
• Visualizing cellular imaging data using PhenoPlot. 

  Sailem, HZ; Sero, JE; Bakal, C (2015-01-08)

  Visualization is essential for data interpretation, hypothesis formulation and communication of results. However, there is a paucity of visualization methods for image-derived data sets generated by high-content analysis ...
• AKT-mTOR signaling modulates the dynamics of IRE1 RNAse activity by regulating ER-mitochondria contacts. 

  Sanchez-Alvarez, M; Del Pozo, MA; Bakal, C (2017-11-28)

  Inositol Requiring Enzyme-1 (IRE1) is the most conserved transducer of the Unfolded Protein Response (UPR), a surveillance mechanism that ensures homeostasis of the endoplasmic reticulum (ER) in eukaryotes. IRE1 activation ...
• Cell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells. 

  Sero, JE; Sailem, HZ; Ardy, RC; Almuttaqi, H; Zhang, T; Bakal, C (2015-03)

  Although a great deal is known about the signaling events that promote nuclear translocation of NF-κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In ...
• TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution. 

  Broderick, R; Nieminuszczy, J; Blackford, AN; Winczura, A; Niedzwiedz, W (2015-03-12)

  During mitosis, sister chromatids must be faithfully segregated to ensure that daughter cells receive one copy of each chromosome. However, following replication they often remain entangled. Topoisomerase IIα (TOP2A) has ...
• Prospective, longitudinal, multi-modal functional imaging for radical chemo-IMRT treatment of locally advanced head and neck cancer: the INSIGHT study. 

  Welsh, L; Panek, R; McQuaid, D; Dunlop, A; Schmidt, M; Riddell, A; Koh, D-M; Doran, S; Murray, I; Du, Y; Chua, S; Hansen, V; Wong, KH; Dean, J; Gulliford, S; Bhide, S; Leach, MO; Nutting, C; Harrington, K; Newbold, K (2015-05-15)

  Radical chemo-radiotherapy (CRT) is an effective organ-sparing treatment option for patients with locally advanced head and neck cancer (LAHNC). Despite advances in treatment for LAHNC, a significant minority of these ...
• Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment. 

  Ip, LRH; Poulogiannis, G; Viciano, FC; Sasaki, J; Kofuji, S; Spanswick, VJ; Hochhauser, D; Hartley, JA; Sasaki, T; Gewinner, CA (2015-04)

  Treatment options for ovarian cancer patients remain limited and overall survival is less than 50% despite recent clinical advances. The lipid phosphatase inositol polyphosphate 4-phosphatase type II (INPP4B) has been ...
• TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation. 

  Blackford, AN; Nieminuszczy, J; Schwab, RA; Galanty, Y; Jackson, SP; Niedzwiedz, W (2015-03)

  The Bloom syndrome helicase BLM and topoisomerase-IIβ-binding protein 1 (TopBP1) are key regulators of genome stability. It was recently proposed that BLM phosphorylation on Ser338 mediates its interaction with TopBP1, to ...
• Direct involvement of retinoblastoma family proteins in DNA repair by non-homologous end-joining. 

  Cook, R; Zoumpoulidou, G; Luczynski, MT; Rieger, S; Moquet, J; Spanswick, VJ; Hartley, JA; Rothkamm, K; Huang, PH; Mittnacht, S (2015-03-26)

  Deficiencies in DNA double-strand break (DSB) repair lead to genetic instability, a recognized cause of cancer initiation and evolution. We report that the retinoblastoma tumor suppressor protein (RB1) is required for DNA ...
• Pten Regulates Epithelial Cytodifferentiation during Prostate Development. 

  Lokody, IB; Francis, JC; Gardiner, JR; Erler, JT; Swain, A (2015-01)

  Gene expression and functional studies have indicated that the molecular programmes involved in prostate development are also active in prostate cancer. PTEN has been implicated in human prostate cancer and is frequently ...
• Removal of H2A.Z by INO80 promotes homologous recombination. 

  Alatwi, HE; Downs, JA (2015-08)

  The mammalian INO80 remodelling complex facilitates homologous recombination (HR), but the mechanism by which it does this is unclear. Budding yeast INO80 can remove H2A.Z/H2B dimers from chromatin and replace them with ...
• Prostate-specific membrane antigen expression in melanoma metastases. 

  Snow, H; Hazell, S; Francis, N; Mohammed, K; O'Neill, S; Davies, E; Mansfield, D; Messiou, C; Hujairi, N; Nicol, D; Harrington, K; Smith, M (2020-06-11)

  BACKGROUND:Prostate-specific membrane antigen (PSMA) is a prostatic epithelial protein that is used as a radiotracer (68Ga-PSMA-11) for prostate cancer staging. PSMA-PET/CT (positron emission tomography/computed tomography) ...
• Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. 

  Hégarat, N; Crncec, A; Suarez Peredo Rodriguez, MF; Echegaray Iturra, F; Gu, Y; Busby, O; Lang, PF; Barr, AR; Bakal, C; Kanemaki, MT; Lamond, AI; Novak, B; Ly, T; Hochegger, H (2020-06)

  Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute ...
• Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. 

  Koundouros, N; Karali, E; Tripp, A; Valle, A; Inglese, P; Perry, NJS; Magee, DJ; Anjomani Virmouni, S; Elder, GA; Tyson, AL; Dória, ML; van Weverwijk, A; Soares, RF; Isacke, CM; Nicholson, JK; Glen, RC; Takats, Z; Poulogiannis, G (2020-06-18)

  Oncogenic transformation is associated with profound changes in cellular metabolism, but whether tracking these can improve disease stratification or influence therapy decision-making is largely unknown. Using the iKnife ...
• The Biochemical Role of the Human NEIL1 and NEIL3 DNA Glycosylases on Model DNA Replication Forks 

  Albelazi; Martin; Mohammed; Mutti; Elder

  <jats:p>Endonuclease VIII-like (NEIL) 1 and 3 proteins eliminate oxidative DNA base damage and psoralen DNA interstrand crosslinks through initiation of base excision repair. Current evidence points to a DNA replication ...
• The Human DNA glycosylases NEIL1 and NEIL3 Excise Psoralen-Induced DNA-DNA Cross-Links in a Four-Stranded DNA Structure 

  Martin, PR; Couvé, S; Zutterling, C; Albelazi, MS; Groisman, R; Matkarimov, BT; Parsons, JL; Elder, RH; Saparbaev, MK (Springer Science and Business Media LLC, 2017-12)
• Combination Therapy With Reovirus and Anti-PD-1 Blockade Controls Tumor Growth Through Innate and Adaptive Immune Responses. 

  Rajani, K; Parrish, C; Kottke, T; Thompson, J; Zaidi, S; Ilett, L; Shim, KG; Diaz, R-M; Pandha, H; Harrington, K; Coffey, M; Melcher, A; Vile, R (2016-02)

  Oncolytic reovirus can be delivered both systemically and intratumorally, in both preclinical models and in early phase clinical trials. Reovirus has direct oncolytic activity against a variety of tumor types and antitumor ...
• Reactivation of p53 by a Cytoskeletal Sensor to Control the Balance Between DNA Damage and Tumor Dissemination. 

  Herraiz, C; Calvo, F; Pandya, P; Cantelli, G; Rodriguez-Hernandez, I; Orgaz, JL; Kang, N; Chu, T; Sahai, E; Sanz-Moreno, V (2016-01)

  BACKGROUND:Abnormal cell migration and invasion underlie metastasis, and actomyosin contractility is a key regulator of tumor invasion. The links between cancer migratory behavior and DNA damage are poorly understood. ...

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