Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.
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Date
2020-06-02ICR Author
Author
Hégarat, N
Crncec, A
Suarez Peredo Rodriguez, MF
Echegaray Iturra, F
Gu, Y
Busby, O
Lang, PF
Barr, AR
Bakal, C
Kanemaki, MT
Lamond, AI
Novak, B
Ly, T
Hochegger, H
Type
Journal Article
Metadata
Show full item recordAbstract
Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis. Loss of cyclin A in G2-phase prevents mitotic entry. Cells lacking cyclin B can enter mitosis and phosphorylate most mitotic proteins, because of parallel PP2A:B55 phosphatase inactivation by Greatwall kinase. The final barrier to mitotic establishment corresponds to nuclear envelope breakdown, which requires a decisive shift in the balance of cyclin-dependent kinase Cdk1 and PP2A:B55 activity. Beyond this point, cyclin B/Cdk1 is essential for phosphorylation of a distinct subset of mitotic Cdk1 substrates that are essential to complete cell division. Our results identify how cyclin A, cyclin B and Greatwall kinase coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phosphorylation.
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Research team
Dynamical Cell Systems
Language
eng
Date accepted
2020-03-25
License start date
2020-06
Citation
The EMBO journal, 2020, 39 (11), pp. e104419 - ?
Publisher
WILEY