Browsing ICR Divisions by author "Pardo Calvo, Maria Mercedes"
Now showing items 21-29 of 29
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Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.
Pardo, M; Yu, L; Shen, S; Tate, P; Bode, D; et al. (NATURE PORTFOLIO, 2017-08-15)MYST histone acetyltransferases have crucial functions in transcription, replication and DNA repair and are hence implicated in development and cancer. Here we characterise Myst2/Kat7/Hbo1 protein interactions in mouse ... -
Nbeal2 interacts with Dock7, Sec16a, and Vac14.
Mayer, L; Jasztal, M; Pardo, M; Aguera de Haro, S; Collins, J; et al. (AMER SOC HEMATOLOGY, 2018-03-01)Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow ... -
Proteomic navigation using proximity-labeling.
Gentzel, M; Pardo, M; Subramaniam, S; Stewart, AF; Choudhary, JS (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019-07-15)The identification of bona fide protein-protein interactions and the mapping of proteomes was greatly enhanced by protein tagging for generic affinity purification methods and analysis by mass spectrometry (AP-MS). The ... -
Regulators of male and female sexual development are critical for the transmission of a malaria parasite.
Russell, AJC; Sanderson, T; Bushell, E; Talman, AM; Anar, B; et al. (CELL PRESS, 2023-02-08)Malaria transmission to mosquitoes requires a developmental switch in asexually dividing blood-stage parasites to sexual reproduction. In Plasmodium berghei, the transcription factor AP2-G is required and sufficient for ... -
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling.
Pardo, M; Bode, D; Yu, L; Choudhary, JS (2017-04)Most proteins act in association with others; hence, it is crucial to characterize these functional units in order to fully understand biological processes. Affinity purification coupled to mass spectrometry (AP-MS) has ... -
The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
Zhang, W; Chronis, C; Chen, X; Zhang, H; Spalinskas, R; et al. (CELL PRESS, 2019-01-03)BAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ... -
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin.
Krastev, DB; Li, S; Sun, Y; Wicks, AJ; Hoslett, G; et al. (NATURE PORTFOLIO, 2022-01-01)Poly (ADP-ribose) polymerase (PARP) inhibitors elicit antitumour activity in homologous recombination-defective cancers by trapping PARP1 in a chromatin-bound state. How cells process trapped PARP1 remains unclear. Using ... -
Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance.
Garcia, LR; Tenev, T; Newman, R; Haich, RO; Liccardi, G; et al. (NATURE PORTFOLIO, 2021-06-07)Necroptosis is a lytic, inflammatory form of cell death that not only contributes to pathogen clearance but can also lead to disease pathogenesis. Necroptosis is triggered by RIPK3-mediated phosphorylation of MLKL, which ... -
UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs.
Gozdecka, M; Meduri, E; Mazan, M; Tzelepis, K; Dudek, M; et al. (NATURE PUBLISHING GROUP, 2018-06-01)The histone H3 Lys27-specific demethylase UTX (or KDM6A) is targeted by loss-of-function mutations in multiple cancers. Here, we demonstrate that UTX suppresses myeloid leukemogenesis through noncatalytic functions, a ...