Nbeal2 interacts with Dock7, Sec16a, and Vac14.
Date
2018-03-01Author
Mayer, L
Jasztal, M
Pardo, M
Aguera de Haro, S
Collins, J
Bariana, TK
Smethurst, PA
Grassi, L
Petersen, R
Nurden, P
Favier, R
Yu, L
Meacham, S
Astle, WJ
Choudhary, J
Yue, WW
Ouwehand, WH
Guerrero, JA
Type
Journal Article
Metadata
Show full item recordAbstract
Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a, and Vac14 as interactors of Nbeal2. We show that GPS-causing mutations in its BEACH domain have profound and possible effects on the interaction with Dock7 and Vac14, respectively. Proximity ligation assays show that these 2 proteins are physically proximal to Nbeal2 in human megakaryocytes. In addition, we demonstrate that Nbeal2 is primarily localized in the cytoplasm and Dock7 on the membrane of or in α-granules. Interestingly, platelets from GPS cases and Nbeal2-/- mice are almost devoid of Dock7, resulting in a profound dysregulation of its signaling pathway, leading to defective actin polymerization, platelet activation, and shape change. This study shows for the first time proteins interacting with Nbeal2 and points to the dysregulation of the canonical signaling pathway of Dock7 as a possible cause of the aberrant formation of platelets in GPS cases and Nbeal2-deficient mice.
Collections
Subject
Animals
Blood Platelets
Blood Proteins
GTPase-Activating Proteins
Guanine Nucleotide Exchange Factors
HEK293 Cells
Humans
Intracellular Signaling Peptides and Proteins
Megakaryocytes
Membrane Proteins
Mice
Mice, Knockout
Mutation
Protein Binding
Vesicular Transport Proteins
Research team
Functional Proteomics
Prote & Metabolomics Fac
Language
eng
Date accepted
2017-11-21
License start date
2018-03-01
Citation
Blood, 2018, 131 (9), pp. 1000 - 1011
Publisher
AMER SOC HEMATOLOGY
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