dc.contributor.author | Mayer, L | |
dc.contributor.author | Jasztal, M | |
dc.contributor.author | Pardo, M | |
dc.contributor.author | Aguera de Haro, S | |
dc.contributor.author | Collins, J | |
dc.contributor.author | Bariana, TK | |
dc.contributor.author | Smethurst, PA | |
dc.contributor.author | Grassi, L | |
dc.contributor.author | Petersen, R | |
dc.contributor.author | Nurden, P | |
dc.contributor.author | Favier, R | |
dc.contributor.author | Yu, L | |
dc.contributor.author | Meacham, S | |
dc.contributor.author | Astle, WJ | |
dc.contributor.author | Choudhary, J | |
dc.contributor.author | Yue, WW | |
dc.contributor.author | Ouwehand, WH | |
dc.contributor.author | Guerrero, JA | |
dc.coverage.spatial | United States | |
dc.date.accessioned | 2023-01-24T13:37:59Z | |
dc.date.available | 2023-01-24T13:37:59Z | |
dc.date.issued | 2018-03-01 | |
dc.identifier | S0006-4971(20)32437-X | |
dc.identifier.citation | Blood, 2018, 131 (9), pp. 1000 - 1011 | |
dc.identifier.issn | 0006-4971 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5661 | |
dc.identifier.eissn | 1528-0020 | |
dc.identifier.eissn | 1528-0020 | |
dc.identifier.doi | 10.1182/blood-2017-08-800359 | |
dc.description.abstract | Mutations in NBEAL2, the gene encoding the scaffolding protein Nbeal2, are causal of gray platelet syndrome (GPS), a rare recessive bleeding disorder characterized by platelets lacking α-granules and progressive marrow fibrosis. We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a, and Vac14 as interactors of Nbeal2. We show that GPS-causing mutations in its BEACH domain have profound and possible effects on the interaction with Dock7 and Vac14, respectively. Proximity ligation assays show that these 2 proteins are physically proximal to Nbeal2 in human megakaryocytes. In addition, we demonstrate that Nbeal2 is primarily localized in the cytoplasm and Dock7 on the membrane of or in α-granules. Interestingly, platelets from GPS cases and Nbeal2-/- mice are almost devoid of Dock7, resulting in a profound dysregulation of its signaling pathway, leading to defective actin polymerization, platelet activation, and shape change. This study shows for the first time proteins interacting with Nbeal2 and points to the dysregulation of the canonical signaling pathway of Dock7 as a possible cause of the aberrant formation of platelets in GPS cases and Nbeal2-deficient mice. | |
dc.format | Print-Electronic | |
dc.format.extent | 1000 - 1011 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | AMER SOC HEMATOLOGY | |
dc.relation.ispartof | Blood | |
dc.rights.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
dc.subject | Animals | |
dc.subject | Blood Platelets | |
dc.subject | Blood Proteins | |
dc.subject | GTPase-Activating Proteins | |
dc.subject | Guanine Nucleotide Exchange Factors | |
dc.subject | HEK293 Cells | |
dc.subject | Humans | |
dc.subject | Intracellular Signaling Peptides and Proteins | |
dc.subject | Megakaryocytes | |
dc.subject | Membrane Proteins | |
dc.subject | Mice | |
dc.subject | Mice, Knockout | |
dc.subject | Mutation | |
dc.subject | Protein Binding | |
dc.subject | Vesicular Transport Proteins | |
dc.title | Nbeal2 interacts with Dock7, Sec16a, and Vac14. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2017-11-21 | |
dc.date.updated | 2023-01-24T13:17:10Z | |
rioxxterms.version | AM | |
rioxxterms.versionofrecord | 10.1182/blood-2017-08-800359 | |
rioxxterms.licenseref.startdate | 2018-03-01 | |
rioxxterms.type | Journal Article/Review | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29187380 | |
pubs.issue | 9 | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Cancer Biology/Functional Proteomics Group | |
pubs.organisational-group | /ICR/ImmNet | |
pubs.publication-status | Published | |
pubs.publisher-url | http://dx.doi.org/10.1182/blood-2017-08-800359 | |
pubs.volume | 131 | |
icr.researchteam | Functional Proteomics | |
icr.researchteam | Prote & Metabolomics Fac | |
dc.contributor.icrauthor | Pardo Calvo, Maria Mercedes | |
dc.contributor.icrauthor | Choudhary, Jyoti | |
icr.provenance | Deposited by Dr Mercedes Pardo Calvo on 2023-01-24. Deposit type is initial. No. of files: 3. Files: Nbeal2 interacts with Dock7, Sec16a, and Vac14.pdf; blood800359-suppl2.cys; blood800359-suppl1.pdf | |