Structural rearrangements of the RNA polymerase III machinery during tRNA transcription initiation.
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Date
2018-04-01ICR Author
Author
Ramsay, EP
Vannini, A
Type
Journal Article
Metadata
Show full item recordAbstract
RNA polymerase III catalyses the synthesis of tRNAs in eukaryotic organisms. Through combined biochemical and structural characterisation, multiple auxiliary factors have been identified alongside RNA Polymerase III as critical in both facilitating and regulating transcription. Together, this machinery forms dynamic multi-protein complexes at tRNA genes which are required for polymerase recruitment, DNA opening and initiation and elongation of the tRNA transcripts. Central to the function of these complexes is their ability to undergo multiple conformational changes and rearrangements that regulate each step. Here, we discuss the available biochemical and structural data on the structural plasticity of multi-protein complexes involved in RNA Polymerase III transcriptional initiation and facilitated re-initiation during tRNA synthesis. Increasingly, structural information is becoming available for RNA polymerase III and its functional complexes, allowing for a deeper understanding of tRNA transcriptional initiation. This article is part of a Special Issue entitled: SI: Regulation of tRNA synthesis and modification in physiological conditions and disease edited by Dr. Boguta Magdalena.
Collections
Subject
Eukaryotic Cells
Animals
Humans
Multiprotein Complexes
RNA Polymerase III
Protein Subunits
Transcription Factors
RNA, Transfer
RNA, Transfer, Amino Acid-Specific
Models, Genetic
Promoter Regions, Genetic
Transcription Elongation, Genetic
Transcription Initiation, Genetic
Research team
Vannini Group
Language
eng
Date accepted
2017-11-11
License start date
2018-04
Citation
Biochimica et biophysica acta. Gene regulatory mechanisms, 2018, 1861 (4), pp. 285 - 294
Publisher
ELSEVIER SCIENCE BV