FDXR is a biomarker of radiation exposure in vivo.
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Date
2018-01-15Author
O'Brien, G
Cruz-Garcia, L
Majewski, M
Grepl, J
Abend, M
Port, M
Tichý, A
Sirak, I
Malkova, A
Donovan, E
Gothard, L
Boyle, S
Somaiah, N
Ainsbury, E
Ponge, L
Slosarek, K
Miszczyk, L
Widlak, P
Green, E
Patel, N
Kudari, M
Gleeson, F
Vinnikov, V
Starenkiy, V
Artiukh, S
Vasyliev, L
Zaman, A
Badie, C
Type
Journal Article
Metadata
Show full item recordAbstract
Previous investigations in gene expression changes in blood after radiation exposure have highlighted its potential to provide biomarkers of exposure. Here, FDXR transcriptional changes in blood were investigated in humans undergoing a range of external radiation exposure procedures covering several orders of magnitude (cardiac fluoroscopy, diagnostic computed tomography (CT)) and treatments (total body and local radiotherapy). Moreover, a method was developed to assess the dose to the blood using physical exposure parameters. FDXR expression was significantly up-regulated 24 hr after radiotherapy in most patients and continuously during the fractionated treatment. Significance was reached even after diagnostic CT 2 hours post-exposure. We further showed that no significant differences in expression were found between ex vivo and in vivo samples from the same patients. Moreover, potential confounding factors such as gender, infection status and anti-oxidants only affect moderately FDXR transcription. Finally, we provided a first in vivo dose-response showing dose-dependency even for very low doses or partial body exposure showing good correlation between physically and biologically assessed doses. In conclusion, we report the remarkable responsiveness of FDXR to ionising radiation at the transcriptional level which, when measured in the right time window, provides accurate in vivo dose estimates.
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Subject
Humans
Neoplasms
Curcumin
Ferredoxin-NADP Reductase
Lipopolysaccharides
RNA
Tomography, X-Ray Computed
Whole-Body Irradiation
Up-Regulation
Adult
Aged
Aged, 80 and over
Middle Aged
Female
Male
Young Adult
Biomarkers
Research team
Translational Breast Radiobiology
Language
eng
Date accepted
2017-12-20
License start date
2018-01-12
Citation
Scientific reports, 2018, 8 (1), pp. 684 - ?
Publisher
NATURE PORTFOLIO