dc.contributor.author | Krastev, DB | |
dc.contributor.author | Pettitt, SJ | |
dc.contributor.author | Campbell, J | |
dc.contributor.author | Song, F | |
dc.contributor.author | Tanos, BE | |
dc.contributor.author | Stoynov, SS | |
dc.contributor.author | Ashworth, A | |
dc.contributor.author | Lord, CJ | |
dc.date.accessioned | 2018-06-05T08:14:15Z | |
dc.date.issued | 2018-05-22 | |
dc.identifier.citation | Nature communications, 2018, 9 (1), pp. 2016 - ? | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/1699 | |
dc.identifier.eissn | 2041-1723 | |
dc.identifier.doi | 10.1038/s41467-018-04466-4 | |
dc.description.abstract | Poly (ADP-ribose)ylation is a dynamic protein modification that regulates multiple cellular processes. Here, we describe a system for identifying and characterizing PARylation events that exploits the ability of a PBZ (PAR-binding zinc finger) protein domain to bind PAR with high-affinity. By linking PBZ domains to bimolecular fluorescent complementation biosensors, we developed fluorescent PAR biosensors that allow the detection of temporal and spatial PARylation events in live cells. Exploiting transposon-mediated recombination, we integrate the PAR biosensor en masse into thousands of protein coding genes in living cells. Using these PAR-biosensor "tagged" cells in a genetic screen we carry out a large-scale identification of PARylation targets. This identifies CTIF (CBP80/CBP20-dependent translation initiation factor) as a novel PARylation target of the tankyrase enzymes in the centrosomal region of cells, which plays a role in the distribution of the centrosomal satellites. | |
dc.format | Electronic | |
dc.format.extent | 2016 - ? | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | |
dc.subject | Cell Line, Tumor | |
dc.subject | Hela Cells | |
dc.subject | Centrosome | |
dc.subject | Centrioles | |
dc.subject | Epithelial Cells | |
dc.subject | Humans | |
dc.subject | Tankyrases | |
dc.subject | Poly Adenosine Diphosphate Ribose | |
dc.subject | Calcium-Binding Proteins | |
dc.subject | Eukaryotic Initiation Factors | |
dc.subject | DNA Transposable Elements | |
dc.subject | Fluorescent Dyes | |
dc.subject | Biosensing Techniques | |
dc.subject | Signal Transduction | |
dc.subject | Mitosis | |
dc.subject | Protein Processing, Post-Translational | |
dc.subject | Recombination, Genetic | |
dc.subject | Genetic Testing | |
dc.subject | Poly ADP Ribosylation | |
dc.title | Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. | |
dc.type | Journal Article | |
dcterms.dateAccepted | 2018-05-01 | |
rioxxterms.versionofrecord | 10.1038/s41467-018-04466-4 | |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0 | |
rioxxterms.licenseref.startdate | 2018-05-22 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | Nature communications | |
pubs.issue | 1 | |
pubs.notes | No embargo | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Gene Function | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Gene Function | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Breast Cancer Research/Gene Function | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Molecular Pathology/Gene Function | |
pubs.publication-status | Published | |
pubs.volume | 9 | |
pubs.embargo.terms | No embargo | |
icr.researchteam | Gene Function | |
dc.contributor.icrauthor | Krastev, Dragomir | |
dc.contributor.icrauthor | Pettitt, Stephen | |
dc.contributor.icrauthor | Campbell, James | |
dc.contributor.icrauthor | Song, Feifei | |
dc.contributor.icrauthor | Lord, Christopher | |