dc.contributor.author | Goyder, MS | |
dc.contributor.author | Willison, KR | |
dc.contributor.author | Klug, DR | |
dc.contributor.author | deMello, AJ | |
dc.contributor.author | Ces, O | |
dc.date.accessioned | 2018-08-08T14:22:00Z | |
dc.date.issued | 2012-04-30 | |
dc.identifier | 4 | |
dc.identifier.citation | BMB REPORTS, 2012, 45 pp. 233 - 238 | |
dc.identifier.issn | 1976-6696 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/2308 | |
dc.identifier.doi | 10.5483/BMBRep.2012.45.4.233 | |
dc.description.abstract | We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome. [BMB reports 2012; 45(4): 233-238] | |
dc.format.extent | 233 - 238 | |
dc.language | eng | |
dc.language.iso | eng | |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | |
dc.title | Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins | |
dc.type | Journal Article | |
rioxxterms.versionofrecord | 10.5483/BMBRep.2012.45.4.233 | |
rioxxterms.licenseref.startdate | 2012-04-30 | |
rioxxterms.type | Journal Article/Review | |
dc.relation.isPartOf | BMB REPORTS | |
pubs.notes | affiliation: deMello, AJ (Reprint Author), ETH, Inst Chem & Biochem Engn, CH-8093 Zurich, Switzerland. deMello, Andrew J., ETH, Inst Chem & Biochem Engn, CH-8093 Zurich, Switzerland. Goyder, Miriam S.; Klug, David R.; Ces, Oscar, Univ London Imperial Coll Sci Technol & Med, Dept Chem, Inst Biol Chem, Single Cell Prote Grp, London SW7 2AZ, England. Willison, Keith R., Inst Canc Res, Chester Beatty Labs, Sect Cell & Mol Biol, London SW3 6JB, England. keywords: Affinity chromatography; Capillary electrophoresis; EVV 2DIR; Proteomics; Ribosomal proteins keywords-plus: FLIGHT MASS-SPECTROMETRY; EXTRARIBOSOMAL FUNCTIONS; EUKARYOTIC RIBOSOME; ESCHERICHIA-COLI; INTACT PROTEINS; IDENTIFICATION; PURIFICATION; MIXTURES; PROTEOME; SUBUNIT research-areas: Biochemistry & Molecular Biology web-of-science-categories: Biochemistry & Molecular Biology author-email: [email protected] [email protected] researcherid-numbers: Arrell, Miriam/D-6461-2016 orcid-numbers: Arrell, Miriam/0000-0003-4658-1309 funding-acknowledgement: EPSRC; BBSRC through the Imperial College London [EP/C54269X/1]; Institute of Chemical Biology Doctoral Training Centre [EP/D505976/1]; Engineering and Physical Sciences Research Council [EP/C54269X/1] funding-text: We gratefully acknowledge funding for this project from the EPSRC and BBSRC through the Single Cell Analysis Project of Imperial College London (EP/C54269X/1) and the Institute of Chemical Biology Doctoral Training Centre (EP/D505976/1). number-of-cited-references: 30 times-cited: 3 usage-count-last-180-days: 0 usage-count-since-2013: 27 journal-iso: BMB Rep. doc-delivery-number: 935BJ unique-id: ISI:000303491700005 oa: gold da: 2018-08-08 | |
pubs.notes | Not known | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation | |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation | |
pubs.volume | 45 | |
pubs.embargo.terms | Not known | |
pubs.oa-location | http://ocean.kisti.re.kr/downfile/volume/ksbmb/E1MBB7/2012/v45n4/E1MBB7_2012_v45n4_233.pdf | |
icr.researchteam | Chromatin Regulation | |
dc.contributor.icrauthor | Willison, Keith | |
icr.provenance | Deposited by Mr Arek Surman (impersonating Keith Willison) on 2022-07-21. Deposit type is subsequent. No. of files: 1. Files: BMB045-04-05.pdf | |