Browsing Closed Research Teams by issue date
Now showing items 1-20 of 467
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Point Mutations in a Hinge Linking the Small and Large Domains of β-Actin Result in Trapped Folding Intermediates Bound to Cytosolic Chaperonin CCT
(2001)The30-Å cryo-EM-derived structure of apo-CCT–α-actin shows actin opened up across its nucleotide-binding cleft and binding to either of two CCT subunit pairs, CCTβ–CCTδ or CCTϵ–CCTδ, in a similar 1:4 arrangement. The two ... -
Mutational Screen Identifies Critical Amino Acid Residues of β-Actin Mediating Interaction between Its Folding Intermediates and Eukaryotic Cytosolic Chaperonin CCT
(2001)Thethree-dimensional reconstruction of apo-CCT-α-actin by cryoelectron microscopy shows that actin binds either the CCTβ–CCTδ or the CCTϵ–CCTδ subunit pairs of the chaperonin in an open and apparently quasi-native conformation. ... -
Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk
(2001)Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk from UK-resident women ... -
Analysis of the Interaction between the Eukaryotic Chaperonin CCT and Its Substrates Actin and Tubulin
(2001)Twomechanisms have thus far been characterized for the assistance by chaperonins of the folding of other proteins. The first and best described is that of the prokaryotic chaperonin GroEL, which interacts with a large ... -
Radiotherapy for brain metastases
(SPRINGER-VERLAG LONDON LTD, 2001) -
Applications of microarray technology in breast cancer research
(BIOMED CENTRAL LTD, 2001)Microarrays provide a versatile platform for utilizing information from the Human Genome Project to benefit human health. This article reviews the ways in which microarray technology may be used in breast cancer research. ... -
The integration of docetaxel into first-line chemotherapy for ovarian cancer.
(2001-01)Docetaxel is being explored as an alternative to paclitaxel in the treatment of ovarian cancer for several reasons: a) evidence of superiority in preclinical models; b) at least comparable activity in platinum-refractory ... -
Differences between blood and liver aromatic DNA adduct formation
(PERGAMON-ELSEVIER SCIENCE LTD, 2001-01)Aromatic DNA adducts in the livers and blood of grey mullet (Mugil sp.) have been monitored between 1993 and 1996 by the isolation of DNA and the postlabeling of the DNA adducts with P-32. Th, grey mullet were sampled from ... -
Randomized trial of porcarbazine, lomustine, and vincristine in the adjuvant treatment of high-grade astrocytoma: A medical research council trial
(2001-01-15)Purpose: Meta-analyses of the published literature suggest ct survival benefit to adjuvant chemotherapy for high-grade astrocytoma, which individual sma trials have been unable to demonstrate reliably. The Medical Research ... -
Multicenter phase II trial of temozolomide in patients with glioblastoma multiforme at first relapse
(KLUWER ACADEMIC PUBL, 2001-02)Background: Recurrent glioblastoma multiforme (GBM) is resistant to most therapeutic endeavors, with low response rates and survival rarely exceeding six months. There are no clearly established chemotherapeutic regimens ... -
Nested allosteric interactions in the cytoplasmic chaperonin containing TCP-1
(2001-02)Initial rates of ATP hydrolysis by the chaperonin containing TCP-1 (CCT) from bovine testis were measured as a function of ATP concentration. Two allosteric transitions are observed: one at relatively low concentrations ... -
Reduced growth in response to ganciclovir treatment of subcutaneous xenografts expressing HSV-tk in the vascular compartment
(2001-06)Reduced growth in response to ganciclovir treatment of subcutaneous xenografts expressing HSV-tk in the vascular compartment. Using a recombinant retrovirus with ecotropic envelope we have achieved high efficiency of ... -
Analyses of DNA adducts formed by ochratoxin A and aristolochic acid in patients with Chinese herbs nephropathy.
(2001-07)Chinese herbs nephropathy (CHN), a unique type of nephropathy has been associated with the intake of weight-reducing pills containing the Chinese herb Aristolochia fangchi. Moreover, an association between the use of A. ... -
Resolution of alpha-hydroxytamoxifen; R-isomer forms more DNA adducts in rat liver cells
(AMER CHEMICAL SOC, 2001-07)The genotoxic tamoxifen metabolite alpha -hydroxytamoxifen has been resolved into R- and S-enantiomers. This was achieved by preparing its ester with S-camphanic acid, chromatographic separation into two diastereoisomers, ... -
N-acetyltransferases, sulfotransferases and heterocyclic amine activation in the breast
(LIPPINCOTT WILLIAMS & WILKINS, 2001-07) -
The ‘sequential allosteric ring’ mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin
(2001-08-01)Folding to completion of actin and tubulin in the eukaryotic cytosol requires their interaction with cytosolic chaperonin CCT [chaperonin containing tailless complex polypeptide 1 (TCP-1)]. Three-dimensional reconstructions ... -
Genotoxicity of human breast milk from different countries
(OXFORD UNIV PRESS, 2001-09)Dietary and/or environmental factors appear to play a key role in the international variations that exist in breast cancer incidence. The genotoxicity of breast milk extracts is being examined as a possible indicator of ... -
Mutability of p53 hotspot codons to benzo(a)pyrene diol epoxide (BPDE) and the frequency of p53 mutations in nontumorous human lung
(2001-09-01)p53 mutations are common in lung cancer. In smoking-associated lung cancer, the occurrence of G:C to TA transversions at hotspot codons, e.g., 157, 248, 249, and 273, has been linked to the presence of carcinogenic chemicals ... -
Psychosocial issues in cancer
(INDIAN ACAD SCIENCES, 2001-09-10)There are many dimensions to psychosocial, issues in cancer; two of which are focused here and relate to (i) the question of whether psychological factors play any role in cancer prognosis, and (ii) the quality of triage ... -
Association between carcinogen-DNA adducts in white blood cells and lung cancer risk in the physicians health study
(AMER ASSOC CANCER RESEARCH, 2001-09-15)In this matched case-control study nested within the prospective Physicians’ Health Study, we evaluated whether DNA damage in blood samples collected at enrollment significantly predicted risk, consistent with our hypothesis ...
