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dc.contributor.authorFournier, Fen_US
dc.contributor.authorGardner, EMen_US
dc.contributor.authorKedra, DAen_US
dc.contributor.authorDonaldson, PMen_US
dc.contributor.authorGuo, Ren_US
dc.contributor.authorButcher, SAen_US
dc.contributor.authorGould, IRen_US
dc.contributor.authorWillison, KRen_US
dc.contributor.authorKlug, DRen_US
dc.date.accessioned2018-08-16T10:38:40Z
dc.date.issued2008-10-07en_US
dc.identifier40en_US
dc.identifier.citationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 pp. 15352 - 15357en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2340
dc.identifier.doi10.1073/pnas.0805127105en_US
dc.description.abstractElectron-vibration-vibration two-dimensional coherent spectroscopy, a variant of 2DIR, is shown to be a useful tool to differentiate a set of 10 proteins based on their amino acid content. Two-dimensional vibrational signatures of amino acid side chains are identified and the corresponding signal strengths used to quantify their levels by using a methyl vibrational feature as an internal reference. With the current apparatus, effective differentiation can be achieved in four to five minutes per protein, and our results suggest that this can be reduced to < 1 min per protein by using the same technology. Finally, we show that absolute quantification of protein levels is relatively straightforward to achieve and discuss the potential of an all-optical high-throughput proteomic platform based on two-dimensional infrared spectroscopic measurements.en_US
dc.format.extent15352 - 15357en_US
dc.languageEnglishen_US
dc.language.isoEnglishen_US
dc.publisherNATL ACAD SCIENCESen_US
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_US
dc.titleProtein identification and quantification by two-dimensional infrared spectroscopy: Implications for an all-optical proteomic platformen_US
dc.typeJournal Article
rioxxterms.versionofrecord10.1073/pnas.0805127105en_US
rioxxterms.licenseref.startdate2008-10-07en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICAen_US
pubs.notesaffiliation: Klug, DR (Reprint Author), Univ London Imperial Coll Sci Technol & Med, Dept Chem, Exhibit Rd, London SW7 2AZ, England. Fournier, Frederic; Gardner, Elizabeth M.; Donaldson, Paul M.; Guo, Rui; Gould, Ian R.; Klug, David R., Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England. Gardner, Elizabeth M.; Donaldson, Paul M.; Gould, Ian R.; Willison, Keith R.; Klug, David R., Univ London Imperial Coll Sci Technol & Med, Chem Biol Ctr, London SW7 2AZ, England. Kedra, Darek A.; Butcher, Sarah A., Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, Bioinformat Support Serv, London SW7 2AZ, England. Willison, Keith R., Ctr Cellular & Mol Biol, Canc Res UK, Chester Beatty Labs, Inst Canc Res, London SW3 6JB, England. keywords: 2DIR; amino acid; bioinformatics; vibrational keywords-plus: AMINO-ACID-COMPOSITION; 4-WAVE-MIXING SPECTROSCOPY; BIOMARKER DISCOVERY; DIAGNOSTICS; PEPTIDES; DATABASE; SPECTRA research-areas: Science & Technology - Other Topics web-of-science-categories: Multidisciplinary Sciences author-email: d.klug@imperial.ac.uk researcherid-numbers: Klug, David/F-3357-2011 Guo, Rui/F-2384-2012 Donaldson, Paul/G-6462-2011 orcid-numbers: Kedra, Darek/0000-0002-9723-4465 gould, ian/0000-0003-3559-0234 funding-acknowledgement: Engineering and Physical Sciences Research Council (EPSRC); Chemical Biology Centre Doctoral Training Centre (CBC DTC); Engineering and Physical Sciences Research Council [EP/C54269X/1, EP/C530187/1, EP/C541839/1] funding-text: We thank Dr. C. J. Barnett for technical support. This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) and the Chemical Biology Centre Doctoral Training Centre (CBC DTC). number-of-cited-references: 28 times-cited: 33 usage-count-last-180-days: 0 usage-count-since-2013: 12 journal-iso: Proc. Natl. Acad. Sci. U. S. A. doc-delivery-number: 364UI unique-id: ISI:000260360500027 oa: gold_or_bronze da: 2018-08-15en_US
pubs.notesNot knownen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Chromatin Regulation
pubs.volume105en_US
pubs.embargo.termsNot knownen_US
icr.researchteamChromatin Regulationen_US
dc.contributor.icrauthorWillison, Keithen_US


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