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dc.contributor.authorTape, CJ
dc.contributor.authorWorboys, JD
dc.contributor.authorSinclair, J
dc.contributor.authorGourlay, R
dc.contributor.authorVogt, J
dc.contributor.authorMcMahon, KM
dc.contributor.authorTrost, M
dc.contributor.authorLauffenburger, DA
dc.contributor.authorLamont, DJ
dc.contributor.authorJørgensen, C
dc.date.accessioned2016-11-23T11:26:59Z
dc.date.issued2014-10-02
dc.identifier.citationAnalytical chemistry, 2014, 86 (20), pp. 10296 - 10302
dc.identifier.issn0003-2700
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/235
dc.identifier.eissn1520-6882
dc.identifier.doi10.1021/ac5025842
dc.description.abstractReproducible, comprehensive phosphopeptide enrichment is essential for studying phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid (45 min), reproducible (r2 ≥ 0.80) and high-fidelity (>90% purity) phosphopeptide purification in a 96-well format. Automated phosphopeptide enrichment demonstrates reproducible synthetic phosphopeptide recovery across 2 orders of magnitude, "well-to-well" quantitative reproducibility indistinguishable to internal SILAC standards, and robust "plate-to-plate" reproducibility across 5 days of independent enrichments. As a result, automated phosphopeptide enrichment enables statistical analysis of label-free phosphoproteomic samples in a high-throughput manner. This technique uses commercially available, off-the-shelf components and can be easily adopted by any laboratory interested in phosphoproteomic analysis. We provide a free downloadable automated phosphopeptide enrichment program to facilitate uniform interlaboratory collaboration and exchange of phosphoproteomic data sets.
dc.formatPrint-Electronic
dc.format.extent10296 - 10302
dc.languageeng
dc.language.isoeng
dc.subjectTitanium
dc.subjectImidazoles
dc.subjectPhosphopeptides
dc.subjectMultivariate Analysis
dc.subjectReproducibility of Results
dc.subjectProteomics
dc.subjectMagnetics
dc.subjectAutomation
dc.titleReproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC.
dc.typeJournal Article
rioxxterms.versionofrecord10.1021/ac5025842
rioxxterms.licenseref.startdate2014-10-02
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfAnalytical chemistry
pubs.issue20
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Cell Communication
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Cell Communication
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene
pubs.publication-statusPublished
pubs.volume86en_US
pubs.embargo.termsNo embargo
icr.researchteamCell Communicationen_US
icr.researchteamOncogeneen_US
dc.contributor.icrauthorTape, Christopheren
dc.contributor.icrauthorWorboys, Jonathan Daviden
dc.contributor.icrauthorJorgensen, Clausen


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