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dc.contributor.authorBettencourt-Dias, M
dc.contributor.authorMittnacht, S
dc.contributor.authorBrockes, JP
dc.date.accessioned2018-09-12T11:45:28Z
dc.date.issued2003-10
dc.identifier.citationJournal of cell science, 2003, 116 (Pt 19), pp. 4001 - 4009
dc.identifier.issn0021-9533
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/2653
dc.identifier.eissn1477-9137
dc.identifier.doi10.1242/jcs.00698
dc.description.abstractAdult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes in culture. We find that the majority of cardiomyocytes can enter S phase, a process that occurs in response to serum-activated pathways and is dependent on the phosphorylation of the retinoblastoma protein. However, more than half of these cells stably arrest at either entry to mitosis or during cytokinesis, thus resembling the behaviour observed in mammalian cardiomyocytes. Approximately a third of the cells progress through mitosis and may enter successive cell divisions. When cardiomyocytes divided more than once, the proliferative behaviour of sister cells was significantly correlated, in terms of whether they underwent a subsequent cell cycle, and if so, the duration of that cycle. These observations suggest a mechanism whereby newt heart regeneration depends on the retention of proliferative potential in a subset of cardiomyocytes. The regulation of the remaining newt cardiomyocytes is similar to that described for their mammalian counterparts, as they arrest during mitosis or cytokinesis. Understanding the nature of this block and why it arises in some but not other newt cardiomyocytes may lead to an augmentation of the regenerative potential in the mammalian heart.
dc.formatPrint-Electronic
dc.format.extent4001 - 4009
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved
dc.subjectMyocardium
dc.subjectMyocytes, Cardiac
dc.subjectAnimals
dc.subjectSalamandridae
dc.subjectRetinoblastoma Protein
dc.subjectRegeneration
dc.subjectCell Division
dc.subjectS Phase
dc.subjectCell Differentiation
dc.subjectPhosphorylation
dc.subjectModels, Biological
dc.titleHeterogeneous proliferative potential in regenerative adult newt cardiomyocytes.
dc.typeJournal Article
rioxxterms.versionofrecord10.1242/jcs.00698
rioxxterms.licenseref.urihttps://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2003-10
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfJournal of cell science
pubs.issuePt 19
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Anti Oncogene
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Anti Oncogene
pubs.publication-statusPublished
pubs.volume116
pubs.embargo.termsNot known
icr.researchteamAnti Oncogeneen_US
dc.contributor.icrauthorMittnacht, Sibylleen


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