Browsing Cancer Biology by author "Bousgouni, Paraskevi"
Now showing items 1-6 of 6
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A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division.
Stojic, L; Lun, ATL; Mascalchi, P; Ernst, C; Redmond, AM; et al. (NATURE PUBLISHING GROUP, 2020-04-15)Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more ... -
ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion.
Bousgouni, V; Inge, O; Robertson, D; Jones, I; Clatworthy, I; et al. (CELL PRESS, 2022-08-19)Rho GTP Exchange Factors (RhoGEFs) and Rho GTPase Activating Proteins (RhoGAPs) are large families of molecules that regulate shape determination in all eukaryotes. In pathologies such as melanoma, RhoGEF and RhoGAP activity ... -
Quantitative imaging of single-cell phenotypes in cancer cells cultured on hydrogel surfaces.
Bousgouni, V; Bakal, C (ELSEVIER, 2023-03-17)Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique ... -
RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer.
Pascual-Vargas, P; Cooper, S; Sero, J; Bousgouni, V; Arias-Garcia, M; et al. (NATURE PUBLISHING GROUP, 2017-03-01)In order to metastasise, triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho Guanine Nucleotide Exchange Factors (RhoGEFs), which activate ... -
Robust deep learning optical autofocus system applied to automated multiwell plate single molecule localization microscopy.
Lightley, J; Görlitz, F; Kumar, S; Kalita, R; Kolbeinsson, A; et al. (WILEY, 2021-06-04)We presenta robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from ... -
WDR5, BRCA1, and BARD1 Co-regulate the DNA Damage Response and Modulate the Mesenchymal-to-Epithelial Transition during Early Reprogramming.
Peñalosa-Ruiz, G; Bousgouni, V; Gerlach, JP; Waarlo, S; van de Ven, JV; et al. (CELL PRESS, 2019-04-09)Differentiated cells are epigenetically stable, but can be reprogrammed to pluripotency by expression of the OSKM transcription factors. Despite significant effort, relatively little is known about the cellular requirements ...