Browsing Cancer Biology by author "Roumeliotis, Theodoros"
Now showing items 1-15 of 15
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An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules.
Bakos, G; Yu, L; Gak, IA; Roumeliotis, TI; Liakopoulos, D; et al. (NATURE PUBLISHING GROUP, 2018-11-14)Covalent modifications of proteins with ubiquitin and ubiquitin-like molecules are instrumental to many biological processes. However, identifying the E3 ligase responsible for these modifications remains a major bottleneck ... -
Aneuploidy tolerance caused by BRG1 loss allows chromosome gains and recovery of fitness.
Schiavoni, F; Zuazua-Villar, P; Roumeliotis, TI; Benstead-Hume, G; Pardo, M; et al. (NATURE PORTFOLIO, 2022-04-01)Aneuploidy results in decreased cellular fitness in many species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth, suggesting that the impact of aneuploidy ... -
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Liess, AKL; Kucerova, A; Schweimer, K; Yu, L; Roumeliotis, TI; et al. (CELL PRESS, 2019-08-06)Ubiquitin-conjugating enzymes (E2s) govern key aspects of ubiquitin signaling. Emerging evidence suggests that the activities of E2s are modulated by posttranslational modifications; the structural underpinnings, however, ... -
Characterization of proteome-size scaling by integrative omics reveals mechanisms of proliferation control in cancer.
Jones, I; Dent, L; Higo, T; Roumeliotis, T; Arias Garcia, M; et al. (AMER ASSOC ADVANCEMENT SCIENCE, 2023-01-25)Almost all living cells maintain size uniformity through successive divisions. Proteins that over and underscale with size can act as rheostats, which regulate cell cycle progression. Using a multiomic strategy, we leveraged ... -
Citrobacter rodentium Subverts ATP Flux and Cholesterol Homeostasis in Intestinal Epithelial Cells In Vivo.
Berger, CN; Crepin, VF; Roumeliotis, TI; Wright, JC; Carson, D; et al. (CELL PRESS, 2017-11-07)The intestinal epithelial cells (IECs) that line the gut form a robust line of defense against ingested pathogens. We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC ... -
Clustering of Tir during enteropathogenic E. coli infection triggers calcium influx-dependent pyroptosis in intestinal epithelial cells.
Zhong, Q; Roumeliotis, TI; Kozik, Z; Cepeda-Molero, M; Fernández, LÁ; et al. (PUBLIC LIBRARY SCIENCE, 2020-12-30)Clustering of the enteropathogenic Escherichia coli (EPEC) type III secretion system (T3SS) effector translocated intimin receptor (Tir) by intimin leads to actin polymerisation and pyroptotic cell death in macrophages. ... -
Genomic Determinants of Protein Abundance Variation in Colorectal Cancer Cells.
Roumeliotis, TI; Williams, SP; Gonçalves, E; Alsinet, C; Del Castillo Velasco-Herrera, M; et al. (CELL PRESS, 2017-08-29)Assessing the impact of genomic alterations on protein networks is fundamental in identifying the mechanisms that shape cancer heterogeneity. We have used isobaric labeling to characterize the proteomic landscapes of 50 ... -
Integrative epigenomics, transcriptomics and proteomics of patient chondrocytes reveal genes and pathways involved in osteoarthritis.
Steinberg, J; Ritchie, GRS; Roumeliotis, TI; Jayasuriya, RL; Clark, MJ; et al. (NATURE PORTFOLIO, 2017-08-21)Osteoarthritis (OA) is a common disease characterized by cartilage degeneration and joint remodeling. The underlying molecular changes underpinning disease progression are incompletely understood. We investigated genes and ... -
Interferon-driven alterations of the host's amino acid metabolism in the pathogenesis of typhoid fever.
Blohmke, CJ; Darton, TC; Jones, C; Suarez, NM; Waddington, CS; et al. (ROCKEFELLER UNIV PRESS, 2016-05-30)Enteric fever, caused by Salmonella enterica serovar Typhi, is an important public health problem in resource-limited settings and, despite decades of research, human responses to the infection are poorly understood. In ... -
Mechanism of assembly, activation and lysine selection by the SIN3B histone deacetylase complex.
Wan, MSM; Muhammad, R; Koliopoulos, MG; Roumeliotis, TI; Choudhary, JS; et al. (NATURE PORTFOLIO, 2023-05-03)Lysine acetylation in histone tails is a key post-translational modification that controls transcription activation. Histone deacetylase complexes remove histone acetylation, thereby repressing transcription and regulating ... -
PBAF loss leads to DNA damage-induced inflammatory signaling through defective G2/M checkpoint maintenance.
Feng, H; Lane, KA; Roumeliotis, TI; Jeggo, PA; Somaiah, N; et al. (COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2022-07-28)The PBRM1 subunit of the PBAF (SWI/SNF) chromatin remodeling complex is mutated in ∼40% of clear cell renal cancers. PBRM1 loss has been implicated in responses to immunotherapy in renal cancer, but the mechanism is unclear. ... -
Proteomic characterisation of triple negative breast cancer cells following CDK4/6 inhibition.
Beykou, M; Arias-Garcia, M; Roumeliotis, TI; Choudhary, JS; Moser, N; et al. (NATURE PORTFOLIO, 2022-07-11)When used in combination with hormone treatment, Palbociclib prolongs progression-free survival of patients with hormone receptor positive breast cancer. Mechanistically, Palbociclib inhibits CDK4/6 activity but the basis ... -
SimPLIT: Simplified Sample Preparation for Large-Scale Isobaric Tagging Proteomics.
Sialana, FJ; Roumeliotis, TI; Bouguenina, H; Chan Wah Hak, L; Wang, H; et al. (AMER CHEMICAL SOC, 2022-08-05)Large scale proteomic profiling of cell lines can reveal molecular signatures attributed to variable genotypes or induced perturbations, enabling proteogenomic associations and elucidation of pharmacological mechanisms of ... -
Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling.
Koliopoulos, MG; Muhammad, R; Roumeliotis, TI; Beuron, F; Choudhary, JS; et al. (NATURE PORTFOLIO, 2022-08-29)Genes encoding the core cell cycle machinery are transcriptionally regulated by the MuvB family of protein complexes in a cell cycle-specific manner. Complexes of MuvB with the transcription factors B-MYB and FOXM1 activate ... -
The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses.
Berger, CN; Crepin, VF; Roumeliotis, TI; Wright, JC; Serafini, N; et al. (PUBLIC LIBRARY SCIENCE, 2018-10-01)Infection with Citrobacter rodentium triggers robust tissue damage repair responses, manifested by secretion of IL-22, in the absence of which mice succumbed to the infection. Of the main hallmarks of C. rodentium infection ...