Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis.
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Date
2018-11-15ICR Author
Author
Lambros, MB
Seed, G
Sumanasuriya, S
Gil, V
Crespo, M
Fontes, M
Chandler, R
Mehra, N
Fowler, G
Ebbs, B
Flohr, P
Miranda, S
Yuan, W
Mackay, A
Ferreira, A
Pereira, R
Bertan, C
Figueiredo, I
Riisnaes, R
Rodrigues, DN
Sharp, A
Goodall, J
Boysen, G
Carreira, S
Bianchini, D
Rescigno, P
Zafeiriou, Z
Hunt, J
Moloney, D
Hamilton, L
Neves, RP
Swennenhuis, J
Andree, K
Stoecklein, NH
Terstappen, LWMM
de Bono, JS
Type
Journal Article
Metadata
Show full item recordAbstract
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635-44. ©2018 AACR.
Collections
Subject
Humans
Prostatic Neoplasms
Cell Transformation, Neoplastic
Blood Component Removal
Cell Count
Cell Separation
In Situ Hybridization, Fluorescence
Genetic Heterogeneity
Male
Neoplastic Cells, Circulating
Comparative Genomic Hybridization
Single-Cell Analysis
High-Throughput Nucleotide Sequencing
Biomarkers, Tumor
Liquid Biopsy
Research team
Cancer Biomarkers
Prostate Cancer Targeted Therapy Group
Translational Therapeutics
Glioma Team
Language
eng
Date accepted
2018-07-18
License start date
2018-11
Citation
Clinical cancer research : an official journal of the American Association for Cancer Research, 2018, 24 (22), pp. 5635 - 5644
Publisher
AMER ASSOC CANCER RESEARCH