Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development.
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Date
2019-01Author
Drosopoulos, K
Linardopoulos, S
Type
Chapter
Metadata
Show full item recordAbstract
Cellular models for siRNA and small molecule high-throughput screening have been widely used in the last decade to identify targets for drug discovery. As an example, we present a twofold readout approach based on cell viability and multipolar phenotype. To maximize the discovery of potential targets and at the same time reduce the number of false positives in our dataset, we have combined focused and rationally designed custom siRNA libraries with small molecule inhibitor libraries. Here we describe a cellular model for centrosome amplification as an example of how to design and perform a multiple readout/multiple screening strategy.
Collections
Subject
Cell Line, Tumor
Centrosome
Animals
Humans
RNA, Small Interfering
Drug Evaluation, Preclinical
Cell Survival
RNA Interference
Gene Library
Small Molecule Libraries
Drug Discovery
High-Throughput Screening Assays
Research team
Drug Target Discovery
Language
eng
License start date
2019-01
Citation
2019, 1953 pp. 33 - 42